包装与食品机械2024，Vol.42Issue(4)：22-26,34,6.DOI:10.3969/j.issn.1005-1295.2024.04.004
白色嗜盐多孢放线菌的筛选以及对聚乙烯保鲜膜的降解
Isolation of Haloactinopolyspora alba and its degradation of polyethylene food preservation film
摘要
Abstract
To solve the problem of difficult degradation of polyethylene(PE)packaging film,a strain from soil that can degrade PE plastic was isolated.After screening and purification,morphological observation,physiological and biochemical identification,and 16S rDNA sequence cloning were done to identify the strain D-1.Then the strain was inoculated on simple inorganic salt medium with PE film as the sole carbon source and cultured at 35℃,150 r/min.The results show that the strain D-1 is identified as Haloactinopolyspora alba.After 28 d incubation,the weight loss of PE film reaches 3.34％.Many holes and fissure were observed on the surface of PE film by SEM;and oxygen-containing functional groups such as carbon-carbon triple bond,ether bond and carboxyl group were introduced into the PE film through infrared scanning,and the laccase activity was 8.7 U/L.This study provides a scientific basis for the degradation of PE film,and enriches the resource bank of the degrading strains.关键词
白色嗜盐多孢放线菌/保鲜膜/聚乙烯/降解/漆酶Key words
Haloactinopolyspora alba/preservation film/polyethylene/degradation/laccase分类
轻工纺织引用本文复制引用
朱利霞,王桂端,陈雅琴,倪雯珂,曾佳璇,李鑫,罗奇..白色嗜盐多孢放线菌的筛选以及对聚乙烯保鲜膜的降解[J].包装与食品机械,2024,42(4):22-26,34,6.基金项目
河南省科技攻关项目(242102320131,222102110057) （242102320131,222102110057）
河南省高等学校重点科研项目(23B180004) （23B180004）
