茶叶科学2024，Vol.44Issue(4)：575-584,10.
不同黄化茶树种质中咖啡碱合成部位的研究
Study on the Synthetic Site of Caffeine in Different Etiolated Tea Germplasms
摘要
Abstract
As the main characteristic metabolite in tea plants,caffeine contributes to tea quality and flavor formation and is a natural functional component. Its function,distribution,biosynthetic pathway and related key genes in tea plants have been basically identified,but its synthetic site at subcellular level needs to be further clarified. In this study,'Baijiguan' and its half-sib offsprings with different etiolated leaves were used as materials. The results of transmission electron microscopy show that the chloroplast structures in etiolated leaves were damaged or destroyed,which was closely related to the SPAD value and leaf phenotype. High performance liquid chromatography (HPLC)was used to determine the caffeine content. It was found that there was still a large amount of caffeine accumulation in etiolated leaves,even more than in normal green leaves. Then,the expression and location of CsTCS1,a key gene involved in caffeine synthesis,were studied by real-time PCR,in-situ hybridization and subcellular localization. It was shown that the expression level of CsTCS1 in different etiolated leaves varied obviously. But the expression site was basically consistent,mainly distributed in the nucleus and cytoplasm of palisade tissues. These results reveal that the synthetic site of caffeine at subcellular level in tea leaves were mainly nucleus and cytoplasm,but not chloroplasts.关键词
茶树/黄化叶片/咖啡碱/合成部位/亚细胞水平Key words
tea plants/etiolated leaf/caffeine/synthetic site/subcellular level分类
农业科技引用本文复制引用
张亚真,钟思彤,陈志辉,孔祥瑞,单睿阳,郑士琴,余文权,陈常颂..不同黄化茶树种质中咖啡碱合成部位的研究[J].茶叶科学,2024,44(4):575-584,10.基金项目
福建省属公益类科研院所基本科研专项(2022R1029002)、国家现代农业产业技术体系(CARS-19)、农业高质量发展超越"5511"协同创新工程(XTCXGC2021004)、福建省农业科学院科技创新团队(CXTD2021006-1) （2022R1029002）
