茶叶科学2024，Vol.44Issue(4)：585-597,13.
茶树蔗糖转运蛋白(SUTs)基因家族鉴定及组织表达分析
Identification and Tissue Expression Analysis of Sucrose Transporter (SUT) Gene Family in Camellia sinensis
摘要
Abstract
Sucrose transporters (SUTs),the main sucrose carriers,consume energy to transport and load sucrose,which play a key role in the transport of plant photosynthetic products from source to sink. In this study,seven members of CsSUTs family were identified from Camellia sinensis 'Shuchazao' by bioinformatics analysis. Their physical and chemical properties,gene structure,subcellular localization,evolutionary relationship and cis-acting elements were analyzed. The identified CsSUT proteins,containing a conserved MFS-2 domain,are closely related to AtSUC proteins in Arabidopsis thaliana,which are clustered in SUTⅠ,Ⅱ and Ⅳ. AtSUC proteins of Arabidopsis thaliana were used as a model in the STRING online website to speculate that there might be a direct interaction between CsSUT proteins and SWEET,SUS and STP proteins. Analysis of the promoter regions of the CsSUT family genes in tea plants reveals that there were masses of cis-acting elements related to hormone response,abiotic stress,and plant growth and development. It is speculated that these promoters may be regulated by plant hormones,stress and other factors,thus affecting the growth and development of tea plants. There were differences in the expression patterns of CsSUT family genes in C. sinensis 'Longjing 43' and C. sinensis 'Shuchazao'. CsSUT6 was highly expressed in flowers,suggesting that it may contribute to the supply,storage and distribution of sucrose in floral organs. CsSUT1 and CsSUT5 were highly expressed in various organs of tea plants,indicating that they may synergistically participate in the process of sucrose loading in ' source' leaves and unloading in 'sink' organs.关键词
茶树/蔗糖转运蛋白/启动子分析/组织特异性/生物信息学分析Key words
Camellia sinensis/sucrose transporters/promoter analysis/tissue specificity/bioinformatics analysis分类
农业科技引用本文复制引用
罗微,张佳琪,杨妮,胡志航,郝建楠,刘慧,谭杉杉,庄静..茶树蔗糖转运蛋白(SUTs)基因家族鉴定及组织表达分析[J].茶叶科学,2024,44(4):585-597,13.基金项目
国家自然科学基金(31870681)、江苏省政策引导类计划(SZ-LYG202126)、江苏园艺种质资源保护和创新计划(JSFEM-202212)、江苏高校优势学科建设项目(PAPD) （31870681）
