茶叶科学2024，Vol.44Issue(4)：627-638,12.
一株云南木霉菌培养条件的优化及其对茶炭疽病的防控研究
Optimization of Culture Conditions of A Trichoderma yunnanensis and Its Control Efficiency of Tea Anthracnose
摘要
Abstract
To optimize the fermentation conditions of Trichoderma yunnanensis,which has antagonistic effect on Colletotrichum camelliae,single factor and response surface tests were performed with the inhibition rate of C.camelliae as the evaluation index. Then,the antagonistic activity,in vitro leaf control effect,indoor pot control effect,and growth effect of the fermentation broth on C. camelliae and tea plants,respectively,were determined. The results show that the optimum fermentation conditions of T. yunnanense were as follows:potato 200 g·L-1,mannitol 18.85 g·L-1,yeast extract 4.73 g·L-1,liquid content 372.60 mL·L-1,culture temperature 25 ℃,pH 6.6,12L∶12D.The inhibitory rate of the obtained fermentation broth on C. Camelliae reached 92.61％ under the ratio of 1∶9 mixed with potato broth (PDA) medium. The control effect of the fermentation broth on anthrax in vitro leaf was 63.71％and that on indoor potted tea plants was 68.95％,both of which were significantly higher than that of T. Harzianum wettable powder and carbendazim. Compared with the water control,the growth of tea seedlings treated with fermentation solution of T. Yunnensis was significantly improved,which was manifested by the increase of root length,root fresh weight,plant height and above ground fresh weight by 69.16％,215.70％,42.13％ and 212.11％ respectively. Overall,T. Yunnanense fermentation liquid has both antibiotic and growth-promoting effects. The results provided a theoretical basis for the application of T. Yunnanense fermentation broth in the biological control of tea anthracnose.关键词
云南木霉菌/茶炭疽病/发酵液/响应面/生物防控Key words
Trichoderma yunnanensis/tea anthracnose/fermentation broth/response surface method/biological control分类
农业科技引用本文复制引用
刘辉,冯月玲,朱秀英,郑周洲,刘思睿,周罗娜,潘雪珍,宋莉..一株云南木霉菌培养条件的优化及其对茶炭疽病的防控研究[J].茶叶科学,2024,44(4):627-638,12.基金项目
贵州省科技支撑计划(黔科合支撑[2021]一般192、黔科合支撑[2023]一般037)、黔南州科技支撑计划(黔南科合[2022]01号) （黔科合支撑[2021]一般192、黔科合支撑[2023]一般037）
