猪伪狂犬病病毒BJC变异株分离鉴定及gB和gE基因序列分析OA北大核心CSTPCD

In order to isolate and identify PRV virant virus in the suspected pseudorabies in the sick pig farm and further determine the molecular characteristics of PRV virant virus,in this study,pathological dissec-tion,isolation and identification,virus purification,ST cells TCID50 and mouse LD50 determination were performed,and the viral gB and gE genes were amplified by PCR,recovered,cloned and sequenced,and the phylogenetic and homology analysis of gB and gE genes were analyzed by bioinformatics analysis software Geneious Prime.The results showed that the isolate was a virulent strain of PRV,the virus grew well in ST cells.After purification,half of the tissue culture infection was determined to be 108.5TCID50/mL,and theLD50 of 6-week-old Kunming mice was 105.8TCID50.The sequencing results were consistent with the expec-ted PRV gB and gE gene fragments.Phylogenetic analysis showed that it was in the same clade as the rep-resentative PRV variants in China,such as JS2012,HN1201,ZJ01,etc.,and was distant from the European and American isolates such as Bartha,Kaplan,Becker,etc.,but was in different large clades.Amino acid se-quence analysis showed that there were multiple characteristic site substitutions between the BJC strain and the foreign classical strain and the early domestic isolated strain,which was consistent with the charac-teristics of the domestic prevalent mutant strain,and the BJC strain was inferred to be a PRV variant strain.