嗜碱芽孢杆菌细胞破碎方法比较及其功效成分分析OA

Extreme microorganisms are a collective term for microorganisms that are most suitable for living in extreme environments,which are rich in a variety of functional components and have broad application prospects in the field of cosmetics.Therefore,it is very important to obtain the active components of extreme microorganisms.This paper aims to develop the active components of extreme microorganisms,in order to provide reliable basis for the development and utilization of alkaliphilic microorganisms in the field of cosmetics.In this experiment,alkaliphilic microorganisms were used to determine the culture conditions by single factor experiment.By using three common disruption methods,the best disruption method of Bacillus alkaliphilic was determined,the functional components of lysing substances were determined,and the antioxidant effect of related components was determined.The experimental results showed that the optimum culture conditions of Bacillus alkaliphilic were as follows:inoculation amount of 3％,culture temperature of 30 ℃,culture time of 26 h,and rotation speed of 180 r/min.By comparing the three common disruption methods,it was concluded that the high-pressure cell disruption method has the best effect,the cell concentration after crushing was the minimum,and the obtained protein content was the highest.In addition,the content of the functional components in the lysosomal substance was determined and the antioxidant effect was determined.The results showed that the contents of polysaccharides,polyphenols and peptides in the lysosomal substance were respectively 2.43 mg/mL,6.27 mg/mL and 0.607 mg/mL.With the increase of the mass fraction of lysosomal substance,the clearance rate of DPPH and ABTS free radicals by Bacillus alkaliphilic gradually increased.The better effect was achieved when the volume fraction was 20％,with the DPPH free radical and ABTS free radical scavenging rates of 43.3％and 37％respectively,and the reducing ability of 33.9％.