核农学报2024，Vol.38Issue(10)：1857-1867,11.DOI:10.11869/j.issn.1000-8551.2024.10.1857
魔芋糖外排转运蛋白基因家族鉴定及表达分析
Genomic Identification and Expression Analysis of Sugars Will Eventually be Exported Transporters Gene Family in Amorphophallus konjac
摘要
Abstract
Sugars will eventually be exported transporters(SWEETs),a new class of energy-independent sugar transporters,play an important role in plant growth.To investigate the role of the AkSWEETs family in the growth of Amorphophallus konjac(A.konjac),we analyzed their physicochemical properties,phylogenetic relationships,gene structure,chromosomal localization,and protein collinearity.Additionally,the expression levels of AkSWEETs in various tissues and during corm growth stages of A.konjac were investigated.Quantitative real-time PCR(qRT-PCR)was utilized to assess the expression of selected AkSWEETs targets following lanthanum induction.The results revealed that 16 AkSWEETs genes were randomly distributed in 10 chromosomes.The proteins encoded by these genes vary in length from 205 to 987 amino acids,with most of the AkSWEETs being alkaline.The AkSWEETs proteins were divided into four subfamilies with distinct functions by phylogenetic analysis,showing close relationships with OsSWEETs proteins.AkSWEETs have gene tandem duplications.Promoter cis-acting element analysis indicated that AkSWEETs contain several cis-elements related to plant hormones,growth,and other functions.Transcriptome data combined with lanthanum-induced qRT-PCR analysis revealed that AkSWEET9/13 play significant roles in regulating both the growth and the accumulation of glucan in the A.konjac corm.These results provide a theoretical reference for further studies on AkSWEETs genes and their biological functions in A.konjac.关键词
魔芋/AkSWEETs/生物信息学/表达分析Key words
Amorphophallus konjac/AkSWEETs/bioinformatics/expression analysis引用本文复制引用
李效贤,冉丹丹,曾周婷,赵佳莹,叶苏婵,岑欣颖,何佳奇,俞振明..魔芋糖外排转运蛋白基因家族鉴定及表达分析[J].核农学报,2024,38(10):1857-1867,11.基金项目
中央本级重大增减支项目(2060302),浙江中医药大学校级科研项目(2020ZG20),国家自然科学基金项目(32000257),浙江省自然科学基金项目(LY23H280003) （2060302）
