金线莲体外抗氧化活性及黄酮醇的合成途径初步研究OA北大核心CSTPCD

To investigate the accumulation pathway of flavonol content in different tissues of Anoectochilus and its relationship with antioxidant properties,the roots,stems,and leaves of Anoectochilus roxburghii(Ar)and Anoectochilus formosanus(Af)were analyzed comprehensively for phenolic content,antioxidant activity,transcriptomics and targeted metabolome.The results showed significant differences among the phenolic content of different tissues of Anoectochilus(leaves>stems>roots),and the total antioxidant analysis showed similar trends.The results of targeted metabolome analysis revealed that flavonol glycosides accounted for the majority of flavonoid compounds in Anoectochilus,especially in the leaves.A total of 166028 non-redundant genes were obtained from roots,stems and leaves transcriptome sequencing,and 30 differentially expressed genes of the flavonol synthesis pathway were screened by comparing eight databases,including KEGG.A weighted gene co-expression network was applied to identify three hub genes,six MYB transcription factors,two O-methyltransferase(OMT)genes,and three uroglucuronide diphosphate glucuronosyltransferase(UGT)genes.The expression patterns of some genes in different tissues were further analysed using quantitative real-time PCR(qRT-PCR),which found that most of the genes are more highly expressed in the Ar.The results of this study provide a basis for the investigation of the molecular synthesis pathway of flavonols in Anoectochilus.