湖泊科学2024，Vol.36Issue(5)：1366-1379,14.DOI:10.18307/2024.0513
冰封期不同类型湖泊细菌与蓝藻群落组成及共现网络分析
Co-occurrence network analysis and community composition of bacteria and cyanobacteria in various types of lakes during frozen period
摘要
Abstract
Lakes in cold climatic zones own unique biological community successions and reciprocal feedbacks between species dur-ing frozen period.As typical lakes in the Inner Mongolia section of the Yellow River Basin,long frozen period provides a natural platform for the study of bacterial and cyanobacterial interactions under frozen conditions.Clarifying such interactions in different lakes can gain a deeper understanding of the functioning and dynamics of the aquatic ecosystems of the lakes under frozen condi-tions.To elucidate the bacteria-algae interactions of different lakes,this paper collected subglacial water samples from Lake Ulansu-hai,Lake Nanhai and Lake Daihai.Bacteria and cyanobacteria and their community structure and relationships between frozen peri-od were investigated using metagenomic sequencing molecular biology technology,redundancy analyses,Mantel test analyses and network analysis models.Results demonstrated that the four major phyla in the three lakes were Proteobacteria,Bacteroidetes,Ac-tinobacteria and Cyanobacteria during the frozen period.The three lakes have significant differences in bacterial and cyanobacterial community composition at the genus level,exhibiting strong relationship with environmental conditions.It is noteworthy that salinity has a significant impact on the microbial composition in Lake Daihai,while ammonia nitrogen concentration imposes great impact on Lake Nanhai.The analysis of the bacteria-cyanobacteria network revealed that more complicated relationship in Lake Nanhai than the other two lakes.More bacteria and algae had competitive antagonistic relationships in Lake Daihai,which is known for its high salinity.Meanwhile,the bacteria-cyanobacteria networks in Lake Daihai and Lake Ulansuhai exhibited higher stability than those in Lake Nanhai.In order to better understand the abiotic factors affecting the co-evolution of bacteria and cyanobacteria,the aim of this paper to sort out the abiotic factors of dominant bacteria and cyanobacteria in the aforementioned three different types of lakes.It also analyzes the significant synergistic relationship between bacteria and cyanobacteria in the three lakes.Finally,it serves as a reference for anticipating and managing lake water issues during frozen period.关键词
冰封期/宏基因/群落结构/冗余分析/菌藻关系Key words
Frozen period/metagenome/community structure/redundancy analysis/bacteria-algae interactions引用本文复制引用
杨文焕,张明宇,杨娅婷,徐龙,郭渊,马杰,李卫平..冰封期不同类型湖泊细菌与蓝藻群落组成及共现网络分析[J].湖泊科学,2024,36(5):1366-1379,14.基金项目
国家自然科学基金项目(42167018)资助. （42167018）
