乳业科学与技术2024，Vol.47Issue(3)：9-15,7.DOI:10.7506/rykxyjs1671-5187-20240505-033
基于实时荧光定量环介导等温扩增技术快速检测发酵乳中嗜热链球菌和保加利亚乳杆菌
Development of Quantitative Loop-Mediated Isothermal Amplification for Rapid Detection of Streptococcus thermophilus and Lactobacillus bulgaricus in Fermented Milk
摘要
Abstract
In this study,a rapid and quantitative method for the detection of viable bacterial counts of Streptococcus thermophilus and Lactobacillus bulgaricus in fermented milk was established using propidium monoazide(PMA)combined with quantitative loop-mediated isothermal amplification(PMA-qLAMP).The PMA treatment conditions for S.thermophilus and L.bulgaricus were optimized.A linear relationship was fitted between the logarithm of bacterial concentration and cycle threshold(Ct)value.The limit of quantification for S.thermophilus and L.bulgaricus was 7.2×103 and 5.6×104 CFU/g,respectively.The PMA-qLAMP method was applied to measure viable bacterial counts in commercial fermented milk,demonstrating good sensitivity and practicality.关键词
发酵乳/环介导等温扩增/叠氮溴化丙锭/乳酸菌Key words
fermented milk/loop-mediated isothermal amplification/propidium monoazide/lactic acid bacteria分类
轻工纺织引用本文复制引用
周帅康,胡连霞,王孟阳,李苗,卢涵,王世杰..基于实时荧光定量环介导等温扩增技术快速检测发酵乳中嗜热链球菌和保加利亚乳杆菌[J].乳业科学与技术,2024,47(3):9-15,7.基金项目
中央引导地方科技发展资金项目(科技创新基地项目)(236Z2804G) （科技创新基地项目）
河北省"三三三人才工程"重点项目(A20231002) （A20231002）
河北省创新能力提升计划项目(22567685H) （22567685H）
石家庄市产业技术研究院建设项目(228790859A) （228790859A）
