乳业科学与技术2024，Vol.47Issue(3)：16-25,10.DOI:10.7506/rykxyjs1671-5187-20240606-041
PRiME MCX固相萃取柱结合超高效液相色谱-串联质谱法测定乳及乳制品中25种β-受体激动剂
Determination of 25 β-Receptor Agonist Residues in Milk and Dairy Products by PRiME MCX Solid Phase Extraction Column Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method for the determination of 25 β-receptor agonist residues in milk and dairy products was established based on ultra-high performance liquid chromatography-tandem mass spectrometry.Samples were enzymatically hydrolyzed in 0.2 mol/L ammonium acetate solution at pH 5.2 for 16 h.After the removal of fat and protein through glass fiber filter paper,the filtrate was purified by solid phase extraction(SPE)using a PRiME MCX solid phase extraction column,and separated on a Waters BEH C18 column(100 mm×2.1 mm,1.7 μm)by gradient elution using a mobile phase composed of acetonitrile and 0.1%formic acid in water.Mass spectrometry was performed using an electrospray ionization source(ESI)in the positive ion mode with multiple reaction monitoring(MRM),and quantitative determination was performed using the internal standard method.The proposed method showed good linearity for all analytes in the concentration range of 0.2-50.0 ng/mL with coefficients of determination(R2)greater than 0.996;the recoveries ranged from 80.3%to 106.2%with relative standard deviations(RSDs)of 0.6%-8.5%(n=6).The limits of detection(LOD)were 0.05-0.10 μg/kg,and the limits of quantification(LOQ)were 0.10-0.20 μg/kg.This method is simple,efficient and suitable for the determination of the 25 β-receptor agonist residues in milk and dairy products.关键词
超高效液相色谱-串联质谱法/乳/乳制品/β-受体激动剂Key words
ultra-high performance liquid chromatography/milk/dairy products/β-receptor agonists分类
轻工纺织引用本文复制引用
范力欣,杨丽琼,任晓伟,杨层层,孟志娟,范素芳..PRiME MCX固相萃取柱结合超高效液相色谱-串联质谱法测定乳及乳制品中25种β-受体激动剂[J].乳业科学与技术,2024,47(3):16-25,10.基金项目
河北省高层次人才资助项目(C20221009) （C20221009）
