水产学报2024，Vol.48Issue(9)：22-35,14.DOI:10.11964/jfc.20231114238
饲料EPA和ALA激活PPARα在吉富罗非鱼脂代谢中的降脂作用
Lipid-lowering effect of PPARα activated by feed EPA and ALA in GIFT Oreochromis niloticus
摘要
Abstract
Dietary fat is an essential nutrient for fish growth,yet the impact of PPARα activation by fatty acids on lipid metabolism in fish is not well understood.This study aimed to investigate the role of PPARα activation and its regulatory effects on lipid metabolism in response to fatty acids in GIFT Oreochromis niloticus using both in vitro and in vivo approaches.Primary hepatocytes from tilapia were incubated with seven individual fatty acids:oleic acid(OA),palmitic acid(PA),alpha-linolenic acid(ALA),linoleic acid(LA),arachidonic acid(AA),eicos-apentaenoic acid(EPA),and docosahexaenoic acid(DHA),and lipid metabolism were measured.ALA,AA and EPA significantly up-regulated the expressions of key fatty acid catabolic genes such as PPARα,carnitine palmitoyltransferase 1(CPT1)and acyl-CoA oxidase(ACO).ALA specifically up-regulated the expressions of lipolysis related genes adipose triglyceride lipase(ATGL),adipose triglyceride lipase(HSL)and fatty acid transport-related genes adipose triglyceride lipase(LPL),respectively.EPA inhibited the expression of ACC,and ALA,EPA and AA could reduce the content of triglycerides in cells.In contrast,PA and OA increased triglyceride content and the number of lipid droplets.In vivo experiments were conducted with two fatty acids,EPA and ALA,which showed distinct gene activation patterns.Six semi-purified diets varying in ALA(0.6％,1.2％)and EPA(0.6％,1.2％)were formulated for normal fat(7％)group,high fat(12％)group(HF)and high fat group with EPA or ALA(0.6％,1.2％),respectively.Tilapia with an initial body weight of(3.53±0.03)g were fed these diets for eight weeks.No significant differences in growth were observed among the groups.However,body fat was signific-antly reduced in all four treatment groups compared to the high-fat group.As ALA and EPA content increased,the condition factor(CF),hepatosomatic index(HSI),and muscle fat index(MFI)decreased,along with serum and liver triglycerides(TG)and cholesterol.No significant differences were found in levels of aspartate aminotrans-ferase(AST),alanine aminotransferase(ALT),superoxide dismutase(SOD),and malondialdehyde(MDA)among the groups.Notably,MDA in the liver was significantly increased in the EPA(1.2％)group,and the expression of PPARα,CPT1,ACO,ATGL and HSL was significantly enhanced with EPA and ALA supplementation.Collect-ively,these findings indicate that diets containing 0.6％EPA or ALA can up-regulate the expression of PPARα and its lipid metabolism-related target genes,leading to a lipid lowering effect.The study's outcomes offer valuable insights for feed formulation and fat source screening.关键词
吉富罗非鱼/PPARα/脂肪酸/降脂效应/离体与在体Key words
GIFT Oreochromis niloticus/PPARα/fatty acids/lipid-lowering effect/in vivo and in vitro分类
农业科技引用本文复制引用
陈森,高敏,杨佳雯,陈晓瑛,吴坤,温小波,荣华,孙育平,宁丽军..饲料EPA和ALA激活PPARα在吉富罗非鱼脂代谢中的降脂作用[J].水产学报,2024,48(9):22-35,14.基金项目
国家自然科学基金(32002400) （32002400）
广州市农业科技特派员项目(2022E04J00253) （2022E04J00253）
养殖罗非鱼肌肉品质参数优化与评估(HXKJHT20221340) National Natural Science Foundation of China(32002400) （HXKJHT20221340）
Guangzhou Agricultural Science and Technology Special Commissioner Project(2022E04J00253) （2022E04J00253）
Optimization and Evaluation of Quality Para-meters in Cultured Tilapia Muscle(HXKJHT20221340) （HXKJHT20221340）
