食品工业科技2024，Vol.45Issue(17)：10-20,11.DOI:10.13386/j.issn1002-0306.2023110189
基于网络药理学及分子对接探究荷叶生物碱抗高尿酸血症的作用机制
Action Mechanism of Nelumbo nucifera Leaf Alkaloids in the Treatment of Hyperuricemia Based on Network Pharmacology and Molecular Docking
摘要
Abstract
Objective:The mechanism of action of Nelumbo nucifera leaf alkaloids in the treatment of hyperuricemia was analyzed based on network pharmacology and molecular docking.Methods:The target of Nelumbo nucifera leaf alkaloids and disease targets related to hyperuricemia were obtained through database search.The"component-disease-target"network of Nelumbo nucifera leaf alkaloids was established through Cytoscape 3.8.0,and the gene ontology(GO)analysis and Kyoto encyclopedia of genes and genome(KEGG)pathway analysis were carried out using the bioinformatics-based platform.The core target and key components were verified by molecular docking.Results:The information of 27 kinds of Nelumbo nucifera leaf alkaloids,including nuciferine,coclaurine,liensinine,was obtained by literature search,and 337 corresponding targets,926 hyperuricemia-related targets and 57 key targets were piedicted.The mechanism of action of Nelumbo nucifera leaf alkaloids in the treatment of hyperuricemia mainly involved GAPDH,STAT3,JUN,CASP3,PTGS2,XDH,MAPK1 and other target genes.These genes mainly played a role in regulating protein expression through AGE-RAGE signaling pathway,PD-L1 expression and PD-1 checkpoint pathway,TNF signaling pathway,IL-17 signaling pathway and p53 signaling pathway.The results of molecular docking showed that the compound had good docking results with the molecular target.Nuciferine,coclaurine and liensinine bound to the key targets spontaneously,among which liensinine formed the most stable structure with PTGS2 and JUN,and the binding energy could reach-9.3 kcal/mol.Conclusion:This study predicted that Nelumbo nucifera leaf alkaloids could ameliorate hyperuricemia by regulating multiple targets and action multiple pathways,which could provide scientific basis for molecular research of Nelumbo nucifera leaf alkaloids in the treatment of hyperuricemia.关键词
网络药理学/荷叶生物碱/高尿酸血症/分子对接/作用机制Key words
network pharmacology/Nelumbo nucifera leaf alkaloids/hyperuricemia/molecular docking/mechanism of action分类
轻工纺织引用本文复制引用
楚邵璇,王晓,唐征,张志毅,董红敬,宫建泉,郑振佳..基于网络药理学及分子对接探究荷叶生物碱抗高尿酸血症的作用机制[J].食品工业科技,2024,45(17):10-20,11.基金项目
山东省重点研发计划(2021CXGC010508) （2021CXGC010508）
百脉山科技研发基金(BMS202301). （BMS202301）
