食品工业科技2024，Vol.45Issue(17)：174-180,7.DOI:10.13386/j.issn1002-0306.2023090197
河套麦胚多肽的制备工艺优化及其体外降血脂活性
Preparation Optimization of Hetao Wheat Germ Polypeptide and Its in Vitro Hypolipidemic Activity
摘要
Abstract
In this paper,alkali protease was used to enzymatically hydrolyze wheat germ proteins grown in the Hetao region.A univariate analysis and an orthogonal experiment were conducted to optimize the enzymatically hydrolyze process conditions of wheat germ proteins,with the degree of hydrolysis taken as the indicator.Moreover,the in vitro hypolipidemic effect of wheat germ polypeptides was investigated.The results showed that the degree of hydrolysis of wheat germ proteins was 37.18％±0.25％achieved by the following conditions,substrate concentration of 6.0％,temperature at 50 ℃,pH of 10.5 and protease amount of 12000 U/g.When the concentration of wheat germ polypeptides was 5 mg/mL,the rate of inhibiting cholesterol solubilisation into micelles was 27.11％±0.36％,the pancreatic lipase activity inhibition rate was 33.54％±0.57％,and cholesterol esterase activity inhibition rate was 38.13％±0.29％.Moreover,after ultrafiltration,the relative molecule weight of polypeptides less than 1 kDa accounted for 54.30％±0.32％of all polypeptides,indicating that low-molecular-weight wheat germ polypeptides might yield superior hypolipidemic effects.The work provides a theoretical foundation for further exploring the mechanism between Hetao wheat germ polypeptides and hypolipidemic effect.关键词
麦胚蛋白/碱性蛋白酶/多肽/降血脂/超滤分离Key words
wheat germ protein/alkaline protease/polypeptide/hypolipidemic/ultrafiltration separation分类
轻工纺织引用本文复制引用
李雨欣,胡芸利,刘聪,边瑞琴,包小兰,王吉力特..河套麦胚多肽的制备工艺优化及其体外降血脂活性[J].食品工业科技,2024,45(17):174-180,7.基金项目
"科技兴蒙"行动重点专项巴彦淖尔国家农业高新技术产业示范区重点项目(NMKJXM202111) （NMKJXM202111）
河套学院科学技术研究项目(HYZX202165) （HYZX202165）
内蒙古自治区自然科学基金资助项目(2021MS03028) （2021MS03028）
巴彦淖尔市科技计划项目(K202105) （K202105）
河套特色农畜产品营养与高值化利用研究中心. （）
