食品工业科技2024，Vol.45Issue(17)：302-315,14.DOI:10.13386/j.issn1002-0306.2023090172
QuEChERS-高效液相色谱三重四级杆线性离子阱串联质谱法测定蔬菜中34种杀虫剂残留
Determination of 34 Insecticide Residues in Vegetables by QuEChERS-High-Performance Liquid Chromatography Triple Quadrupole Linear Ion Trap Tandem Mass Spectrometry
摘要
Abstract
This article established a rapid detection method for 34 pesticides in vegetables by combining QuEChERS purification method with high-performance liquid chromatography triple quadrupole linear ion trap tandem mass spectrometry.The samples were extracted with acetonitrile(containing 1％acetic acid),1.5 g sodium acetate,4 g anhydrous magnesium sulfate,and 1 g sodium chloride.The extract was further purified using 20 mg graphitized carbon black(GCB),300 mg primary secondary amine(PSA),200 mg octadecylsilane chemically bonded silica(C18),and 1000 mg MgSO4.Acetonitrile-water was chosen as mobile phase.The samples were analyzed using MRM-IDA-EPI mode,and an EPI database was established.Qualitative analysis was performed based on retention time,ion pair peak ratio,and EPI database,and quantification was carried out using matrix-matched external standard method.The study results indicated that using eggplant,garlic chives,cowpea,and spinach as representative matrices,the 34 pesticides showed good linear correlation with R2≥ 0.99011.The detection limits ranged from 0.16 to 1.81 μg/kg,and the quantification limits ranged from 0.52 to 6.02 μg/kg.The spiked recoveries of the samples were between 73.51％and 118.77％,and the precision was in the range of 0.01％to 14.27％.Vegetable samples were tested,with a detection rate of 58.23％for thiamethoxam.This method was characterized by its rapid and effective detection,simple operation,accurate data,and high sensitivity,making it suitable for the detection of 34 pesticides in vegetables.关键词
杀虫剂/高效液相色谱三重四级杆线性离子阱串联质谱/蔬菜/QuEChERSKey words
insecticide/high-performance liquid chromatography-triple quadrupole linear ion trap tandem mass spectrometry/vegetables/QuEChERS分类
轻工纺织引用本文复制引用
罗景阳,李娇,关健,李巧莲,王岩松,袁帅..QuEChERS-高效液相色谱三重四级杆线性离子阱串联质谱法测定蔬菜中34种杀虫剂残留[J].食品工业科技,2024,45(17):302-315,14.基金项目
辽宁省市场监督管理局科技计划项目(2022ZC023) （2022ZC023）
沈阳市科技计划项目(22-322-3-37). （22-322-3-37）
