食品工业科技2024，Vol.45Issue(17)：327-335,9.DOI:10.13386/j.issn1002-0306.2023090323
基于Fe-HHTP@ZIF-8构建可视化传感器测定饮料的总抗氧化能力
Colorimetric Biosensor Based on Fe-HHTP@ZIF-8 for the Detection of Total Antioxidant Capacity in Beverages
摘要
Abstract
Total antioxidant capacity of food is one of the critical parameters to evaluate its efficacy.Establishment of a fast and sensitive analytical method for the detection of total antioxidant capacity of food has great significance.In this paper,a metal-organic framework complex nanozyme(Fe-HHTP@ZIF-8)with peroxidase-like activity was firstly designed and successfully synthesized.Then,the morphology,structure and catalytic activity were characterized in detail.The results showed that Fe-HHTP@ZIF-8 possessed a two-dimensional ultra-thin layer structure while its Km and Vmax values towards H2O2 were 0.67 mmol/L and 3.55×10-8 mol/L·s-1,respectively.Ascorbic acid(AA)as a typical antioxidant,an efficient colorimetric biosensor was constructed based on its inhibitory effect on chromogenic reaction catalyzed by nanozyme.Comprehensive validation showed that the colorimetric biosensor had low limit of detection(1.67 μmol/L),wide linear range(5.0～3000.0 μmol/L)and high accuracy(recovery rate of 98.62％～103.13％).By using this colorimetric method,total antioxidant capacity of different beverages was detected and the obtained results were basically consistent with labeled values.Therefore,this study provided a simple,fast and effective colorimetric method for the evaluation of the total antioxidant capacity of food.关键词
总抗氧化能力/比色检测/过氧化物模拟酶Key words
total antioxidant capacity/colorimetric detection/peroxidase-like nanozyme分类
轻工纺织引用本文复制引用
武存慧,秦之阳,张海枝..基于Fe-HHTP@ZIF-8构建可视化传感器测定饮料的总抗氧化能力[J].食品工业科技,2024,45(17):327-335,9.基金项目
国家自然科学基金项目(32302220) （32302220）
湖北省教育厅项目(T2022023). （T2022023）
