食品科学2024，Vol.45Issue(17)：9-16,8.DOI:10.7506/spkx1002-6630-20230719-204
红毛藻多糖抑制大肠杆菌黏附Caco-2细胞的机制
Inhibitory Mechanism of Escherichia coli Adhesion to Caco-2 Monolayers by Polysaccharides from Bangia fusco-purpurea
摘要
Abstract
In this study,the effect of Bangia fusco-purpurea polysaccharide(BFP)on Escherichia coli adhesion to Caco-2 monolayers and its potential mechanism was studied using an in vitro human colon adenocarcinoma cell(Caco-2)monolayer model.The effect of BFP on the adhesion of E.coli to Caco-2 monolayers was analyzed by fluorescence labeling with carboxyfluorescein diacetate and succinimidyl ester(CFDA-SE),and its effect on the gene expression of integrin β1 in Caco-2 cells,the adhesin FimH in E.coli,and inflammatory factors(IL-1β,IL-8 and TNF-α)induced by E.coli adhesion to Caco-2 cells and the tight junction proteins zonula occludens 1(ZO-1)and occludin were analyzed using real-time polymerase chain reaction(real-time PCR).Also,the protein expression of ZO-1 and occludin in Caco-2 monolayers was detected using Western Blot analysis.Our results showed that BFP at concentrations of 400-800 μg/mL significantly inhibited the adhesion of E.coli to Caco-2 monolayers mainly by down-regulating the gene expression of integrin β1 in Caco-2 cells and FimH in E.coli.In addition,BFP significantly inhibited the up-regulation of inflammatory cytokine gene expression and the down-regulation of ZO-1 and occludin protein and gene expression induced by in Caco-2 cells induced by E.coli and its culture supernatant.In conclusion,BFP inhibited the adhesion of E.coli to Caco-2 cell monolayers,which will lay a scientific basis for the development of novel antimicrobial products and the high-value utilization and deep processing of BFP.关键词
红毛藻多糖/大肠杆菌/Caco-2细胞/细菌黏附/肠道稳态Key words
Bangia fusco-purpurea polysaccharide/Escherichia coli/Caco-2 cells/bacterial adhesion/intestinal homeostasis分类
轻工业引用本文复制引用
段舒舒,郑明静,洪涛,朱艳冰,倪辉,姜泽东..红毛藻多糖抑制大肠杆菌黏附Caco-2细胞的机制[J].食品科学,2024,45(17):9-16,8.基金项目
国家自然科学基金面上项目(31972081) （31972081）
福建省科技计划引导性项目(2023N0013) （2023N0013）
