食品科学2024，Vol.45Issue(17)：42-51,10.DOI:10.7506/spkx1002-6630-20231027-238
总状蕨藻盾叶变种寡糖的酶解制备及其免疫调节活性的作用机制
Enzymatic Preparation and Immunomodulatory Mechanism of Oligosaccharide from Caulerpa racemosa var.peltate
摘要
Abstract
In this study,oligosaccharide from Caulerpa racemosa var.peltate(CRVO-G)was prepared by sequential enzymatic hydrolysis with lactase,membrane filtration,dialysis,and freeze-drying,and the relative molecular mass and monosaccharide composition of CRVO-G were measured.Meanwhile,the immunoregulatory activity of CRVO-G was evaluated and its mechanism of action was studied.The results showed that CRVO-G was a mixture of two tetrasaccharides and one hexasaccharide with relative molecular masses of 617.115,621.081,and 995.368,and was mainly composed of galactose(51.0％),mannose(21.3％),glucose(11.5％),xylose(6.5％),rhamnose(2.3％),glucuronic acid(2.0％),glucosamine hydrochloride(1.8％),fucose(0.8％),glucosamine hydrochloride(0.7％),galacturonic acid(0.7％),mannuronic acid(0.7％),and arabinose(0.6％).In addition,CRVO-G had good immunomodulatory activity,as manifested by increased release of nitric oxide and cytokines(interleukin(IL)-1β,IL-6 and tumor necrosis factor(TNF)-α).The results of metabolomics analysis and Western blot showed that CRVO-G could promote the synthesis of thromboxane A2(TXA2)and 15-keto prostaglandin F2α(15-keto-PGF2α)in the arachidonic acid pathway of macrophages,and increase the expression levels of cyclooxygenase-2(COX-2),inducible nitric oxide synthase(iNOS),and the phosphorylation of nuclear factor κB(NF-κB).In summary,CRVO-G exerts immunomodulatory effects by the NF-KB/iNOS/COX-2 pathway in RAW264.7 macrophages.关键词
总状蕨藻盾叶变种/寡糖/酶解/免疫调节/分子机制Key words
Caulerpa racemosa var.peltate/oligosaccharide/enzymatic hydrolysis/immunomodulatory/molecular mechanism分类
轻工业引用本文复制引用
曾瑾子,林倩敏,许静宜,徐宝军,黄耀南,黄日明..总状蕨藻盾叶变种寡糖的酶解制备及其免疫调节活性的作用机制[J].食品科学,2024,45(17):42-51,10.基金项目
国家自然科学基金国际(地区)合作与交流项目(32161160304) （地区）
澳门科学技术发展基金与国家自然科学基金委员会联合资助项目(0069/2021/AFJ) （0069/2021/AFJ）
