食品科学2024，Vol.45Issue(17)：71-79,9.DOI:10.7506/spkx1002-6630-20231215-123
亚油酸诱导植物乳杆菌p-8产生共轭亚油酸的蛋白组和类组蛋白乙酰化分析
Proteome and Histone-Like Acetylation Analysis of Conjugated Linoleic Acid Production by Lactobacillus plantarum p-8 Induced by Linoleic Acid
摘要
Abstract
In this study,changes in proteome and histone-like acetylation levels of Lactobacillus plantarum p-8 induced by linoleic acid(LA)were explored to investigate the mechanism of conjugated linoleic acid(CLA)production.The results showed that the optimal concentration of LA for inducing CLA production was 1 mg/mL.Proteomic analysis revealed that at this concentration,key protein factors for fatty acid synthesis such as enoyl acyl carrier protein(ACP)reductase and acyl carrier proteins were down-regulated,fatty acid synthesis was down-regulated,and acetyltransferase was up-regulated.Western blot showed that the increase in the content of acetyl-CoA upon NaAc addition resulted in elevated histone-like protein acetylation levels.The addition of LA and NaAc increased the mRNA expression of CLA-related enzymes,transcription factors(TF)and Sigma factors,as determined by real-time polymerase chain reaction(real-time PCR),and increased the transformation rate of CLA.Correlation analysis yielded a significant positive correlation between the transcriptional levels of acetyl-CoA and linoleic acid hydratase(CLA-hy)and the transformation rate of CLA,revealing that the up-regulation of acetyl-CoA and acetyltransferase contents led to enhanced histone-like acetylation and consequent up-regulation of CLA synthesis.These results provide a basis for molecular modification and scientific regulation of lactic acid bacteria to improve CLA conversion.关键词
亚油酸/共轭亚油酸/植物乳杆菌p-8/蛋白组/类组蛋白乙酰化Key words
linoleic acid/conjugated linoleic acid/Lactobacillus plantarum p-8/proteome/histone-like protein acetylation分类
轻工业引用本文复制引用
李瑞华,秦雅丽,董其格其,张和平,赵国芬..亚油酸诱导植物乳杆菌p-8产生共轭亚油酸的蛋白组和类组蛋白乙酰化分析[J].食品科学,2024,45(17):71-79,9.基金项目
内蒙古自然科学基金项目(2023MS03047 （）
2023LHMS0) （）
"饲用作物及有益微生物种质资源创新与分子育种"团队项目(TD202103) （TD202103）
内蒙古自治区研究生科研创新项目(S20231113Z) （S20231113Z）
