食品科学2024，Vol.45Issue(17)：96-104,9.DOI:10.7506/spkx1002-6630-20240122-196
基于RAW264.7细胞模型的槲皮万寿菊素与叶黄素协同改善急性肺损伤的作用机制
Synergistic Ameliorative Effect of Quercetagetin and Lutein on Acute Lung Injury in RAW264.7 Cells
摘要
Abstract
This study aimed to elucidate the ameliorative effect and mechanism of quercetagetin,quercetin and lutein,alone and in combination,on acute lung injury.An lipopolysaccharide-induced inflammation model was established based on RAW264.7 cells.The optimal mixture ratios between quercetagetin or quercetin and lutein were determined based on the content of nitric oxide(NO)in RAW264.7 cells using combination index(CI)analysis.The individual and combined effects of quercetagetin,quercetin and lutein on the contents of malondialdehyde(MDA),inflammatory cytokines(including tumor necrosis factor α(TNF-α),interleukin(IL)-1β and IL-6)and the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)in LPS-stimulated RAW264.7 cells were investigated.In addition,the relative expression levels of p65,p50,silent information regulator 1(SIRT1),nuclear factor-erythroid 2 related factor 2(Nrf2),and NOD-like receptor thermal protein domain associated protein 3(NLRP3)were evaluated by Western blot.The results showed that the lowest NO level in RAW264.7 cells was observed by treatment with a mixture of high-dose quercetagetin and lutein of 3∶1(30 μg/mL+10 μg/mL).Quercetagetin,lutein and their combination all decreased the contents of MDA and inflammatory cytokines,increased the activities of SOD and GSH-PX,down-regulated the expression of nuclear factor kappa-B(NF-κB)p65,p50 and NLRP3,and up-regulated the expression of SIRT1 and Nrf2,the combination being more effective than either treatment alone.关键词
万寿菊/槲皮万寿菊素/叶黄素/槲皮素/RAW264.7细胞/急性肺损伤Key words
marigold(Tagetes erecta L.)/quercetagetin/lutein/quercetin/RAW264.7 cells/acute lung injury分类
轻工纺织引用本文复制引用
冯丹琦,王向红,吴迪,连运河,程鑫颖,刘卫华,米思..基于RAW264.7细胞模型的槲皮万寿菊素与叶黄素协同改善急性肺损伤的作用机制[J].食品科学,2024,45(17):96-104,9.基金项目
"十四五"国家重点研发计划重点专项(2022YFE0124900) （2022YFE0124900）
国家自然科学基金青年科学基金项目(32202013) （32202013）
河北省自然科学基金生物农业联合基金培育项目(C2022204222) （C2022204222）
