食品科学2024,Vol.45Issue(17):216-225,10.DOI:10.7506/spkx1002-6630-20231226-218
近冰温贮藏在延缓甜龙竹笋采后木质化衰老中的作用
Effect of Near-Freezing Temperature Storage on Delaying Postharvest Lignification of Dendrocalamus brandisii Shoots
摘要
Abstract
In this study,the regulatory effect of near-freezing temperature(NFT)storage on postharvest lignification of Dendrocalamus brandisii shoots was evaluated.Samples stored at room temperature and 4 ℃ served as controls.Respiratory intensity,mass loss rate,hardness,H2O2 content,lignin content,and the activities and gene expression of key enzymes involved in lignin synthesis were measured during storage.The results showed that compared with the controls,NFT storage significantly inhibited the increase of respiration intensity,mass loss,hardness,H2O2 content and lignin content in D.brandisii shoots,and delayed the increase of phenylalanine ammonia-lyase(PAL),cinnamyl alcohol dehydrogenase(CAD),cinnamate 4-hydroxylase(C4H),and peroxidase(POD)activities.Transcriptomic analysis showed that 33 genes members in PAL,4CL,HCT,CCR,and CAD families were down-regulated and 25 gene members were up-regulated.Correlation analysis revealed that most physiological indexes and the activities and gene expression of key enzymes involved in lignin synthesis were significantly and positively correlated with the lignin content.In conclusion,NFT storage could effectively suppress the lignification of D.brandisii shoots during storage by regulating the respiratory intensity,mass loss rate,hardness,H2O2 content,lignin content,and key enzyme activities and gene expression related to lignin synthesis.关键词
竹笋/近冰温贮藏/木质化/转录组/采后Key words
bamboo shoots/near-freezing temperature storage/lignification/transcriptome/postharvest分类
轻工纺织引用本文复制引用
杨双鹤,董霞,于丽娟,申挥,罗海波,苏火生,邹琴,俞源河,陈亚男,余元善,胡腾根..近冰温贮藏在延缓甜龙竹笋采后木质化衰老中的作用[J].食品科学,2024,45(17):216-225,10.基金项目
云南省重大科技专项(202202AE090023) (202202AE090023)