食品科学2024，Vol.45Issue(18)：87-98,12.DOI:10.7506/spkx1002-6630-20231021-167
辣木籽凝乳酶在水牛乳干酪制备中应用及产血管紧张素转化酶抑制肽分析
Application of Moringa oleifera Seed Rennet in the Preparation of Buffalo Cheese:Optimization of Processing Technology and Mining of Angiotensin Converting Enzyme Inhibitory Peptides
摘要
Abstract
In this study,buffalo cheese was prepared with rennet from Moringa oleifera seeds.The process was optimized based on sensory characteristics and hydrolysis degree.The composition of water-soluble peptides from the cheese was analyzed.Furthermore,bioactive peptides with angiotensin converting enzyme(ACE)inhibitory activity were identified and their potential molecular mechanisms of action were explored.The optimal conditions determined were as follows:pH 5.56,rennet dose 0.14%,incubation time 32 min,and stretching temperature 80℃.Under these conditions,the hydrolysis degree of cheese was 12.56%with a uniform and elastic texture,a soft and delicate taste,a uniform and glossy color,and a rich aroma.By liquid chromatography-tandem mass spectrometry(LC-MS/MS),1 600 peptides with molecular mass less than 3 kDa were detected in the cheese,613 of which demonstrated good bioactivity,predominately derived from β-casein and αS1-casein.Notably,37.29%of the peptides exhibited ACE inhibitory activity.By comparing with the bioactive peptide database and using bioinformatics,four peptides proven to have ACE inhibitory activity and four novel peptides with potential ACE inhibitory activity were identified.Molecular docking showed that the four novel peptides could effectively bind to ACE.Among them,FGGL and FSPL bound tightly to the S2 active pocket,thus exhibiting strong ACE inhibitory activity.This study provides a scientific basis for the development and utilization of new plant rennet resources and characteristic buffalo cheese.关键词
辣木籽凝乳酶/水牛乳干酪/液相色谱-串联质谱/血管紧张素转化酶抑制肽/分子对接Key words
Moringa oleifera seed rennet/buffalo cheese/liquid chromatography-tandem mass spectrometry/angiotensin converting enzyme inhibitory peptides/molecular docking分类
轻工业引用本文复制引用
钟玉旺,于梦怡,张丹,常莹,幸福兵,黄艾祥,王雪峰..辣木籽凝乳酶在水牛乳干酪制备中应用及产血管紧张素转化酶抑制肽分析[J].食品科学,2024,45(18):87-98,12.基金项目
国家自然科学基金地区科学基金项目(32360562) （32360562）
云南省农业联合专项重点项目(202101BD070001-013) （202101BD070001-013）
云南省科学技术协会2022-2024年度青年科技人才托举工程项目 （）
