食品科学2024，Vol.45Issue(18)：350-360,11.DOI:10.7506/spkx1002-6630-20231012-098
数字聚合酶链式反应技术在食品安全核酸检测领域中的研究进展及标准化现状
Progress in the Application of Digital Polymerase Chain Reaction in Food Safety Detection and Current Status of Its Standardization
摘要
Abstract
Digital polymerase chain reaction(dPCR)is a novel nucleic acid amplification technology that enables absolute quantitative analysis of nucleic acid at the single-molecule level through extreme dilution and Poisson distribution statistics without any need for internal reference genes or standard curves.It has the advantages of high sensitivity,accuracy and tolerance,and has great development potential in the field of food safety detection.This paper introduces the technical principle,advantages and disadvantages,and commercial platforms of dPCR,and summarizes recent progress in the application of dPCR in the fields of foodborne pathogen detection,animal-and plant-derived component detection,genetically modified component detection,and foodborne virus detection and the current status of the standardization of dPCR.The problems existing in this technology and possible future development directions are discussed.We hope that this review can provide ideas for promoting the application of dPCR in food safety detection and formulating a standard for dPCR.关键词
数字聚合酶链式反应/食品安全/标准化Key words
digital polymerase chain reaction/food safety/standardization分类
轻工纺织引用本文复制引用
秦爱,王娟,邓方进,余秋地,周朝旭,李根容,肖昭竞..数字聚合酶链式反应技术在食品安全核酸检测领域中的研究进展及标准化现状[J].食品科学,2024,45(18):350-360,11.基金项目
重庆市市场监督管理局科研计划项目(CQSJKJ2021039) （CQSJKJ2021039）
