产碱性蛋白酶地衣芽孢杆菌合成培养基筛选与优化OA北大核心CSTPCD
Screening and optimization of chemically defined medium for alkaline protease production by Bacillus licheniformis
该研究对于产碱性蛋白酶地衣芽孢杆菌(Bacillus licheniformis)的合成培养基进行了系统的筛选与优化.基于豆粕成分分析及文献报道构建初始化学合成培养基CDM0,并通过单一组分移除实验,初步确定对菌体生长有显著影响(生长降低20％以上)的组分,包括3种碱基、4种维生素、11种氨基酸以及一些金属离子和无机盐.在此基础上进行了组分添加实验,分别添加单一组分移除实验从CDM.中剔除的组分,进而筛选出对菌体生长具有20％以上促进作用的5种关…查看全部>>
In this study,a systematic screening and optimization approach was employed to identify and characterize the optimal chemically defined medium for cultivating Bacillus licheniformis to produce alkaline protease.The initial chemically defined medium CDM0 was constructed based on the analysis of the composition of soybean meal and relevant literature reviews.The components that significantly affected the growth of the bacterium(growth reduced by more than 20％)…查看全部>>
姚巧儿;张英;王永红
华东理工大学,生物反应器工程国家重点实验室,上海,200237华东理工大学,生物反应器工程国家重点实验室,上海,200237华东理工大学,生物反应器工程国家重点实验室,上海,200237
地衣芽孢杆菌碱性蛋白酶化学合成培养基筛选优化碳源
Bacillus licheniformisalkaline proteasechemically defined mediumscreening and optimizationcarbon source
《食品与发酵工业》 2024 (16)
30-39,10
国家重点研发计划(2021YFC2100205)
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