食品与发酵工业2024，Vol.50Issue(16)：235-241,7.DOI:10.13995/j.cnki.11-1802/ts.037522
太白贝母挥发油提取的工艺优化及其成分分析
Research on extraction process and chemical composition of volatile oil of Fritillaria taipaiensis P.Y.Li
摘要
Abstract
To elucidate the main components of volatile oil in Fritillaria taipaiensis P.Y.Li,the yield of volatile oil of F.taipaiensis(％)was utilized as the investigation index,whilst the material-liquid ratio,extraction time,and extraction temperature comprised the in-vestigation factors.Single-factor and Box-Behnken response surface design experiments were implemented to optimize the optimal extraction process for the volatile oil of F.taipaiensis.The extraction process was then coupled with GC-MS and used in combination with the NIST20.L standard spectrum library to search and identify the chemical composition of the sample.Additionally,the peak area normaliza-tion method was employed to determine the relative percentage content of each component.Results showed that the optimal conditions for maximum yield of F.taipaiensis volatile oil could be achieved with a material-liquid ratio(g∶mL)of 1∶12,an extraction time of 52 min,and an extraction temperature of 48 ℃,thereby reaching a yield of 0.365％.The GC-MS analysis identified a total of 41 compounds,with predominant components such as palmitic acid,linoleic acid,and myristic acid.The classes of identified compounds were primarily acids(47.94％),alcohols(9.59％),and esters(7.26％).The highest percentage of components found were palmitic acid(11.17％),lin-oleic acid(8.64％),cis,cis-9,12-octadecadienol(5.68％),pentadecanoic acid(4.57％),and so on.The optimized extraction process is simple,fast,and stable,and can be used for the extraction of volatile oil components in F.taipaiensis.The findings of this research of-fer valuable theoretical insights into the comprehensive development and utilization of F.taipaiensis resources.关键词
太白贝母/挥发油/Box-behnken设计/超声辅助提取/GC-MSKey words
Fritillaria taipaiensis P.Y.Li/volatile oil/Box-Behnken design/ultrasonic assisted extraction/GC-MS引用本文复制引用
姚欢,江春阳,王悦珩,罗娅,陶敬,郭冬琴,周浓..太白贝母挥发油提取的工艺优化及其成分分析[J].食品与发酵工业,2024,50(16):235-241,7.基金项目
重庆市教育委员会自然科学基金重点项目(KJZD-K202101201) （KJZD-K202101201）
重庆市万州区科技创新项目(wzstc20210216) （wzstc20210216）
