摘要
Abstract
Perilla seed oil contains rich and active ingredients such as a-linolenic acid,tocopherols,phytosterols,and polyphenols.Studying the effects of Perilla seed oil on the antioxidant activity and intestinal microorganisms can deliver valuable information for its de-velopment and application.Fifty KM male mice were randomly divided into 5 groups,including blank group,model group,and Perilla seed oil groups.The Perilla seed oil groups consisted of low dose group(1.25 mL/kg BW),medium dose group(2.5 mL/kg BW),and high dose group(5 mL/kg BW).The feces of the blank group and Perilla seed oil groups were collected for intestinal microflora sequen-cing after intervention for 30 days.The effects of Perilla seed oil on the intestinal microorganisms of mice were studied by detecting micro-bial diversity,species abundance,and differential changes in different mice groups.An ethanol oxidative damage model was established,and the effects of Perilla seed oil on the antioxidant capacity of mice were evaluated by measuring the growth performance indexes and the contents of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione(GSH),and protein carbonyl(PCO).Compared with the blank group,the results showed that the amount of fecal microbiota of the Perilla seed oil mice group increased,and the species changed significantly,indicating that Perilla seed oil had effects of weight loss and antioxidant stress.Compared with the ethanol oxidative damage model group,the PCO and MDA were significantly reduced in Perilla seed oil mice group,the SOD was significantly increased in the medium and high dose Perilla seed oil mice groups,GSH was increased,the mice weight was significantly reduced,and the visceral-body ratio of heart and kidney were improved in high dose Perilla seed oil mice group.In conclusion,Perilla seed oil can regulate the in-testinal flora of mice,improve anti-oxidation capacity,and slightly enhance the weight loss efficiency.关键词
紫苏籽油/小鼠生长性能/乙醇氧化损伤/抗氧化能力/微生物多样性Key words
Perilla seed oil/growth performance of mice/oxidative damage model of ethanol/antioxidant capacity/microbial diversity
