食品与发酵工业2024，Vol.50Issue(16)：285-292,8.DOI:10.13995/j.cnki.11-1802/ts.039458
蜂蜜中链霉素仿生印迹电化学传感检测方法
Biomimetic imprinted electrochemical sensing detection of streptomycin in honey
摘要
Abstract
There has been a serious hazard for streptomycin residues in animal-derived foods.It is necessary to establish a high-per-formance analytical method to ensure food safety and human health.The biomimetic imprinting electrochemical sensing detection method has the advantages of high efficiency,specificity,and sensitivity,and has become an important area of research in the field of food safety detection technology.In this study,a biomimetic imprinted sensing interface was constructed by electropolymerization with streptomycin as the template,graphite oxide and chitosan as the sensitizing materials,and 3-aminobenzeneboronic acid and O-phenylenediamine as the composite functional monomers.The biomimetic imprinted electrochemical sensing detection method was then developed and applied for STR residue analysis in honey.The imprinted interface was characterized by scanning electron microscopy,and the electrochemical per-formance of the proposed sensor was evaluated with cyclic voltammetry,differential pulse voltammetry,and electrochemical impedance a-nalysis.Under the optimal reaction conditions,the linear range was calculated as 1.0 × 10-10-1.0 × 10-6 mol/L with a detection limit of 2.59 × 10-12 mol/L,the recoveries of sophora nectar,jujube nectar,and osmanthus nectar were 94.78％-106.24％,93.65％-103.81％,and 97.19％-106.18％,respectively,and the relative standard deviation was less than or equal to 4.28％.The experimental results show that this method has good repeatability,stability,and reproducibility.The related research provides technical support for the high specificity and ultrasensitive analysis of trace target compounds in complex matrices,which is of great significance for the rapid screening and safety monitoring of streptomycin in honey.关键词
蜂蜜/链霉素/仿生印迹/电化学传感器Key words
honey/streptomycin/biomimetic imprinting/electrochemical sensor引用本文复制引用
李兆周,于慧春,袁云霞,吴影,李佩艳,赵明辉,张冉,陈秀金,王耀,万宁波,牛华伟,高红丽,李芳..蜂蜜中链霉素仿生印迹电化学传感检测方法[J].食品与发酵工业,2024,50(16):285-292,8.基金项目
洛阳市公益性行业科研专项(2202021A) （2202021A）
河南省科技攻关项目(242102411001,232102320298) （242102411001,232102320298）
河南省优秀青年科学基金(202300410121) （202300410121）
国家自然科学基金(31701694,U1504330,31702218) （31701694,U1504330,31702218）
河南省青年人才托举工程项目(2020HYTP029) （2020HYTP029）
河南省研究生教育改革与质量提升工程项目(HNYJS2020JD06) （HNYJS2020JD06）
