食品与发酵工业2024，Vol.50Issue(16)：299-305,7.DOI:10.13995/j.cnki.11-1802/ts.038145
扩增子测序技术在牡蛎GⅡ型诺如病毒基因多样性研究中的评估
Evaluation of genetic diversity of GⅡ noroviruses in oyster by amplicon sequencing technology
摘要
Abstract
The exploration of norovirus genetic diversity within oysters is of paramount importance in understanding the virus's epi-demic spread and devising effective prevention and control strategies.Amplicon sequencing,a next-generation sequencing(NGS)tech-nique,offers significant advantages,such as high throughput and accuracy,commonly applied in genomics,transcriptomics,and epig-enomics.This study aims to assess the viability of amplicon sequencing technology for probing norovirus diversity in oysters.To evaluate its efficacy,oyster samples were intentionally contaminated with norovirus underwent amplicon sequencing using the classical nested RT-PCR method.The analysis aimed to ascertain the viral genotype coverage and sensitivity of this approach.Results demonstrated that amplicon se-quencing successfully detected all six GⅢ noroviruses present in oysters,achieving 100％coverage.Remarkably,the technique's sensitiv-ity was underscored through the detection and sequencing analysis of oysters contaminated with varying amounts of noroviruses,confirming its ability to identify less than 50 viral particles.In summary,amplicon sequencing technology markedly enhances the accuracy and geno-typic richness of norovirus detection in oysters.关键词
诺如病毒/贝类/扩增子测序/引物评估/多样性Key words
norovirus/shellfish/amplicon sequencing/primer evaluation/diversity引用本文复制引用
赵璐,董蕾,喻勇新,靳淼,王永杰..扩增子测序技术在牡蛎GⅡ型诺如病毒基因多样性研究中的评估[J].食品与发酵工业,2024,50(16):299-305,7.基金项目
国家自然科学基金(31601570) （31601570）
上海市科学技术委员会项目(22 N31900700) （22 N31900700）
徐州市重点研发计划(社会发展)(KC21296) （社会发展）
