食品与发酵工业2024，Vol.50Issue(16)：332-340,9.DOI:10.13995/j.cnki.11-1802/ts.036240
基于超高效液相色谱串联质谱法和网络药理学探究雨生红球藻提取物延缓皮肤衰老机制
Study on mechanism of Haematococcus pluvialis extract in skin age-delaying based on UPLC-MS/MS and network pharmacology
唐荣 1孙锦月 1李钧翔 2何聪芬1
作者信息
- 1. 北京工商大学化学与材料工程学院,北京,100048
- 2. 浙江清华长三角研究院衰老科学创新研发中心,浙江嘉兴,314006||禾美生物科技有限公司,浙江嘉兴,314006
- 折叠
摘要
Abstract
This study aimed to identify the active ingredient of Haematococcus pluvialis extract(HPE)by UPLC-MS/MS,to predict the mechanism of HPE in delaying skin aging by network pharmacology and to verify the mechanism experimentally.Based on TCMSP,SwissTargetPrediction,CellAge,and other databases that collect the intersection targets of HPE and aging,the protein-protein interaction networks were used to screen the core targets and key components,and gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed on the core targets.Results showed that there were 16 active ingredients of HPE,corresponding to 196 targets,and the protein-protein interaction networks screened 30 core targets,corresponding to 14 key ingredients.GO enrichment analysis and KEGG pathway enrichment analysis revealed the regulatory activity of HPE to inflammation through mitogen-activated protein kinase signaling,transient receptor potential ion channel mediator regulation,nuclear factor kappa-B signaling,etc.Molecular docking was used to predict the binding ability of key components to the core targets,and the docking scores showed good binding ability between the key components and the core targets.Furthermore,in vitro experiments showed that HPE could significantly inhibit the expression of inflammatory factors in mouse mononuclear macrophage cells and increase the collagen I level of hu-man foreskin fibroblast cells upon ultraviolet A irradiation.HPE may delay skin aging through the regulation of inflammatory pathways and inflammatory factors and have multi-component-multi-target-multi-pathway characteristics.关键词
雨生红球藻提取物/UPLC-MS/MS/网络药理学/皮肤衰老Key words
Haematococcus pluvialis extract/UPLC-MS/MS/network pharmacology/skin aging引用本文复制引用
唐荣,孙锦月,李钧翔,何聪芬..基于超高效液相色谱串联质谱法和网络药理学探究雨生红球藻提取物延缓皮肤衰老机制[J].食品与发酵工业,2024,50(16):332-340,9.
