犊牛腹泻症的病原学检测及鉴定OA北大核心CSTPCD

[Objective]This article aims to identify the main pathogens of calf diarrhea in a large-scale cattle farm in Xinjiang.[Methods]Virus detection was performed on 14 diarrhea calf feces using RT-PCR.Besides,bacteria were isolated from the liver tissues of four dead calves,cultured and identified,and mouse pathogenicity and drug sensitivity tests were conducted on these bacteria.Afterwards,the 16 S rDNA gene fragment of the isolated pathogenic bacteria was amplified and sequenced,and the sequencing results were searched for homologous sequences using BLAST in NCBI for genetic evolution analysis.[Results]The patho-gens of diarrhea in the cattle farm were Bovine coronavirus(BCoV),Bovine rotavirus(BRV),Escherichia co-li(E.coli),and Klebsiella pneumoniae(K.pneumoniae).Among the 14 feces,the detection rate of BCoV was 64.29%,and the detection rate of BRV was 50.00%;Among the four liver tissues,the detection rate of E.coli was 100.00%,and the detection rate of K.pneumoniae was 50.00%.The two isolated pathogenic bacteria E.coli and K.pneumoniae were sensitive to amikacin and resistant to all 16 other antibiotics.[Con-clusion]The main pathogens causing calf diarrhea in this cattle farm are BCoV,BRV,E.coli,and K.pneu-moniae,with mixed infection of viruses and bacteria.Amikacin can be used as the preferred drug for the pre-vention and treatment of E.coli and K.pneumoniae.