畜牧与兽医2024，Vol.56Issue(9)：28-32,5.
棕榈粕低聚糖对体外乳酸杆菌生长及其抑菌性能的影响
The effect of palmkernel cake oligosaccharides on the proliferation and antibacterial performance of Lactobacillus was evaluated using an in vitro method
摘要
Abstract
This experiment was to explore the effect of palm kernel cake oligosaccharides(PKCOS)on the proliferation and antibacterial function of lactic acid bacteria in vitro,so as to provide some theoretical basis and data reference for the scientific compatibility of innovative probiotics and prebiotics,as well as the development of high-quality synbiotic products.The candidate probiotics were determined according to the number of viable bacteria,pH and OD600 nm from the 7 strains of Lactobacillus retained in the laboratory,and they were cultured in dif-ferent proportions(1%,3%,5%,7%)with PKCOS instead of glucose as carbon sources.Then,the antibacterial effects of the candidate probiotics cultured in three groups,including the sugar-free group,the whole glucose group,and the 3%PKCOS substitution group,on Escherichia coli and Staphylococcus aureus were explored.The results were as follows:L.plantarum LY19 was a candidate probiotic.The opti-mal substitution ratio of PKCOS for glucose culture L.plantarum LY19 was 3%.Compared with the sugar-free group,in the whole glucose group and the 3%PKCOS substitution group,the value of viable bacteria was also 9.35,and the lactate yield was 120 mg/mL,but there were no significant difference between the two groups.Compared with the whole glucose group,the inhibition zone of L.plantarum LY19 against Escherichia coli and S.aureus was significantly increased after 24 h of culture in the 3%PKCOS substitution group.In conclusion,when 3%PKCOS replaced glucose as the carbon source,the antibacterial function of L.plantarum LY19 on E.coli and S.aureus can be en-hanced.关键词
棕榈粕低聚糖/大肠杆菌/金色葡萄球菌/植物乳酸杆菌/体外研究Key words
palm kernel cake oligosaccharides/Escherichia coli/Staphylococcus aureus/Lactobacillus plantarum/in vitro studies分类
农业科技引用本文复制引用
曾慧,廖经宏,杭苏琴..棕榈粕低聚糖对体外乳酸杆菌生长及其抑菌性能的影响[J].畜牧与兽医,2024,56(9):28-32,5.基金项目
国家重点研发计划项目子课题项目(2021YFD1300-301-5) （2021YFD1300-301-5）
