中国兽医科学2024，Vol.54Issue(9)：1137-1148,12.DOI:10.16656/j.issn.1673-4696.2024.0147
STING agonist 22免疫增强剂配伍ASFV P30蛋白对猪免疫效果的评价
Evaluation of the immune efficacy of STING agonist 22 immune enhancer combined with ASFV P30 protein in pigs
摘要
Abstract
This study evaluated the immune effect of ASFV P30 target protein in pigs using STING ag-onist 22 as a candidate immune enhancer,and provided a preliminary experimental basis for screening the immunity booster of African swine fever(ASF)subunit vaccine.The P30 structural protein with high immunogenicity of prokaryotic expression of ASFV in our laboratory was selected as the target antigen and combined with STING agonist 22 immune enhancer and ISA 201VG adjuvant dosed with ASFV P30 protein for 2 immunization test pigs to compare the enhancement effect of STING agonist 22 immune enhancer in inducing cellular immunity,humoral immunity,and antigen delivery response.The results showed that the addition of STING agonist 22 immune enhancer group immunized test pigs significantly advanced the time of P30-mediated antibody response,increased the expression level of B cell maturation and differ-entiation-related transcription factors,and promoted the maturation of DCs and enhanced their antigen delivery,compared with 201VG adjuvant dosing alone.The results of this study showed that STING agonist 22 immune enhancer paired with ASFV P30 protein could significantly enhance cellular and humoral im-mune responses and induce stronger antigen presentation in pigs,and could be used as an immune enhancer for ASF subunit vaccines.关键词
非洲猪瘟病毒/STING agonist 22/免疫增强剂/树突状细胞Key words
African swine fever virus/STING agonist 22/immune enhancer/dendritic cells分类
农业科技引用本文复制引用
彭勤,曹伟军,杨帆,刘华南,茹毅,朱紫祥,郑海学,张桂红..STING agonist 22免疫增强剂配伍ASFV P30蛋白对猪免疫效果的评价[J].中国兽医科学,2024,54(9):1137-1148,12.基金项目
国家重点研发计划项目(2021YFD1801300,2021YFD1800100) （2021YFD1801300,2021YFD1800100）
中国农业科学院基础科学中心重大任务(CAAS-CSLPDCP-202302) （CAAS-CSLPDCP-202302）
国家生猪产业体系项目(CARS-35) （CARS-35）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
