中国药理学通报2024，Vol.40Issue(9)：1680-1687,8.DOI:10.12360/CPB202401014
新基因C12ORF56在肺癌中的表达特征和功能研究
Expression pattern and function of novel gene C12ORF56 in lung cancer
摘要
Abstract
Aim To disclose the subcellular localiza-tion,expression pattern,cellular physiological function and possible molecular mechanism of C12ORF56,a novel gene located at q14.2 of chromosome 12,in the pathogenesis of lung cancer.Methods ONCOMINE database was applied to investigate the mRNA level dif-fering of C12ORF56 between normal and lung cancer tissues.Analysis based on LinkedOmics,Metascape,String and GSEA database or tools provided indication of potential cellular physiological functions of C12ORF56 in the developing of lung cancer.C12ORF56 was knocked down via siRNA and the pro-liferation of NCI-H1073 cells were observed by EdU and CCK-8 assay.RT-qPCR was used to detect the ex-pression level of C12ORF56 of lung cancer cells on dif-ferent cycle phases.The core sequence regions of pro-moter affecting the transcription of C12ORF56 gene were analyzed by Jaspar online-tools and verified by dual-luciferase assay.Results C12ORF56 was highly expressed in lung cancer cells,especially in squamous cell lung cancer.C12ORF56 correlated with cell cy-cle,cancer immune,DNA replication.Knockdown of C12ORF56 reduced NCI-H1703 cell proliferation.Conclusion The up-regulation of C12ORF56 is in-volved in the development of lung cancer by enhancing lung cancer cell proliferation.关键词
C12ORF56基因/肺癌/鳞状细胞肺癌/细胞增殖/基因功能预测/表达特征Key words
C12ORF56 gene/lung cancer/squamous cell lung carcinoma/cell proliferation/gene function prediction/expression pattern分类
基础医学引用本文复制引用
张增,徐华珍,秦润禾,黄蕴敏,凌亚豪,昌文林..新基因C12ORF56在肺癌中的表达特征和功能研究[J].中国药理学通报,2024,40(9):1680-1687,8.基金项目
广东省自然科学基金面上项目(No 2021A1515010515) （No 2021A1515010515）
深圳市龙华区医疗卫生机构区级科研项目(No 2021029) （No 2021029）
