中国药理学通报2024，Vol.40Issue(9)：1735-1743,9.DOI:10.12360/CPB202404039
原花青素B2通过NRF2/HO-1/xCT/GPX4轴抑制氧化应激减轻H2O2诱导的人少突胶质细胞的损伤
Proanthocyanin B2 inhibits oxidative stress and alleviates H2O2 induced damage to human oligodendrocytes through NRF2/HO-1/xCT/GPX4 axis
摘要
Abstract
Aim To explore the protective effect of an-thocyanin B2(PCB2)on hydrogen peroxide(H2O2)induced oxidative damage and apoptosis in human oli-godendrocytes(MO3.13)and the underlying mecha-nism.Methods The optimal concentration of H2O2 and PCB2 for action was screened,and divided into normal group,PCB2 group(100 mg·L-1 PCB2 treat-ment for 24 hours),H2 O2 model group(500 μmol·L-1 H2O2 treatment for 24 hours),and H2O2+PCB2 group(500 μmol·L-1 H2O2 and 100 mg·L-1 PCB2 co-treated for 24 hours).FRAP method was used to detect the antioxidant capacity of PCB2;CCK-8 meth-od was used to detect the survival rate of cells in each group,while LDH method was used to assess cytotoxic-ity.Microenzyme-linked immunosorbent assay and ELISA were used to examine the levels of LDH,NO,H2O2,as well as the activities of CAT and SOD in each group of cells.Immunofluorescence and Western blot were used to detect the protein expression levels of NRF2,xCT,HO-1,ferritin,and GPX4 in each group of cells.FerroOrange fluorescent probe was used to de-tect the intracellular content of ferrous ions(Fe2+).Results H2O2 could induce MO3.13 oxidative dam-age and lead to cell ferroptosis,while PCB2 could alle-viate MO3.13 oxidative damage and ferroptosis.Com-pared with the H2O2 model group,PCB2 intervention could significantly increase LDH content in MO3.13,reduce NO and H2O2 content,and improve SOD and CAT activity,and up-regulate the protein expression levels of NRF2,xCT,HO-1,ferritin,and GPX4.Conclusion PCB2 can enhance cellular antioxidant capacity and alleviate H2O2 induced MO3.13 oxidative damage through the NRF2/HO-1/xCT/GPX4 axis.关键词
原花青素B2/MO3.13/H2O2/氧化损伤/NRF2/HO-1/xCT/GPX4轴/铁死亡Key words
proanthocyanin B2/MO3.13/H2O2/ox-idative damage/NRF2/HO-1/xCT/GPX4 axis/ferrop-tosis分类
中医学引用本文复制引用
刘健,王青,陈莹,梁亚杰,蒲萌,张紫薇,郑璐璐,柴智,肖莹,马存根..原花青素B2通过NRF2/HO-1/xCT/GPX4轴抑制氧化应激减轻H2O2诱导的人少突胶质细胞的损伤[J].中国药理学通报,2024,40(9):1735-1743,9.基金项目
国家青年科学基金资助项目(No 81903596) （No 81903596）
山西省回国留学人员科研资助项目(No 2022-165) （No 2022-165）
国家中医药管理局科研课题(No 2023ZYYDA2038) （No 2023ZYYDA2038）
山西省卫健委医学科技领军团队(No 2020TD05) （No 2020TD05）
山西省中医药管理局科研课题(No 2023ZYYB040) （No 2023ZYYB040）
山西省卫健委2022年度中医药科研课题立项计划(No 2022ZYYC090) （No 2022ZYYC090）
山西中医药大学学科建设经费(No 2024XKJS-02) （No 2024XKJS-02）
山西省教育厅科技创新项目(No 2022L336) （No 2022L336）
山西中医药大学2022年度科技创新团队(No 2022TD2006) （No 2022TD2006）
山西中医药大学科技创新能力培育计划(No 2022-TY-PH-11,2022-TY-PH-37) （No 2022-TY-PH-11,2022-TY-PH-37）
