中外葡萄与葡萄酒Issue(5)：14-21,8.DOI:10.13414/j.cnki.zwpp.2024.05.002
胚挽救创制玫瑰香型无核葡萄新种质研究
Research on Cultivating New Seedless Grape Germplasm with Muscat Flavor by Embryo Rescue
摘要
Abstract
To create new germplasm of seedless grapes with muscat flavor,seven cross combinations were conducted,involving four stenospermocarpic seedless grape varieties'Ruby Seedless''Aishen Meigui''Huozhou Ziyu'and'Wuhe Cuibao',with the male parents being the varieties'Jumeigui''Aishen Meigui''Shine Muscat'and'Muscat Hamburg',respectively.The effects of different female and male parents on embryo development rate,germination rate,and seedling formation rate were compared.The marker-assisted selection on the hybrids using the seedless gene molecular marker VviAGL11-KASP and the muscat flavor gene molecular marker VviDXS-KASP were used to identify hybrid parents and assist in the selection of hybrid offsprings.The results showed that'Ruby Seedless'was suitable as the female parent for embryo rescue.'Shine Muscat'and'Muscat Hamburg'were more suitable as male parents than'Jumeigui'and'Aishen Meigui'when'Ruby Seedless'was used as the female parent.A total of 316 hybrid strains of seven cross combinations were obtained by embryo rescue,out of which 176 strains had a seedless marker,with an average seedless rate of 55.70%,183 strains had a muscat flavor marker,with the ratio of muscat flavor was 57.91%.There were 110 strains with seedless and muscat flavor markers,accounting for 34.81%.关键词
无核葡萄/胚挽救/玫瑰香型/亲本基因型/分子标记Key words
seedless grape/embryo rescue/muscat flavor/parental genotype/molecular marker分类
农业科技引用本文复制引用
崔小月,尚泓泉,吕中伟,娄玉穗,张柯,樊红杰,吴文莹..胚挽救创制玫瑰香型无核葡萄新种质研究[J].中外葡萄与葡萄酒,2024,(5):14-21,8.基金项目
财政部和农业农村部:国家现代农业产业技术体系项目(CARS-29-19)河南省中央引导地方科技发展资金项目(Z20231811173) （CARS-29-19）
河南省农业科学院自主创新项目(2024ZC030) （2024ZC030）
