阳春砂茉莉酸生物合成酶基因鉴定与时空表达分析OA北大核心CSTPCD

To explore the relationship between the expression of jasmonic acid(JA)biosynthetic genes and the growth of stamen and pistil of A.villosum.The full-length JA biosynthase genes were cloned using RACE technology,and their sequences were analyzed using the bioinformatics method.The gene expressions and their correlation with stamen and pistil growth were then examined.The results showed that in A.villosum,the full lengths of the JA biosynthase genes AvLOX,AvAOS,AvAOC,and AvOPR3 were 2 733 bp,1 798 bp,930 bp,and 1 400 bp respectively,with ORFs of 2 577 bp,1 485 bp,744 bp,and 1 179 bp,encoding 858,494,247,and 392 amino acids.The expressions of these four JA biosynthetic enzyme genes increased as the stamen developed in A.villosum,however,there was diverse expressions in the pistil.In pistil,the expressions of AvLOX and AvAOS peaked on the first day after flowering.The expression peak of AvAOC occurred on the 9th day after marking,and the expression peak of AvOPR3 occurred on the 6th day after marking.Futhermore,the expressions of these four JA biosynthase genes in stamen were significantly higher than those in pistil at the same developmental stage,except for AvAOS on the 3rd day and AvOPR3 on the 6th day in floweret.Compared to the control,the expression patterns for four JA biosynthase genes in stamen,as well as AvLOX and AvOPR3 in pistil,remained unchanged after spraying MeJA(methyl jasmonate).However,there were differences observed for both AvAOS and AvAOC in the pistil.Under both the control and MeJA treatments,the expressions of four JA synthase genes showed significant correlations with the length of the filament and style within A.villosum floweret.This study lays a foundation for further research on the regulation of JA on the growth and development of the floral organ of A.villosum.