肉类研究2024，Vol.38Issue(8)：42-47,6.DOI:10.7506/rlyj1001-8123-20240531-130
基于离子对竞争性结合作用检测牛肉中16种氨基糖苷类兽药残留
Detection of 16 Aminoglycoside Residues in Beef Based on Ion Pair Competitive Binding
摘要
Abstract
A method was established for simultaneous determination of 16 aminoglycoside(AG)residues in beef.Samples were extracted with phosphate buffered solution and the proteins were precipitated using trichloroacetic acid.After adjusting the pH,the target compounds were cleaned up on an HLB solid phase extraction(SPE)column,separated on a Hilic Plus column,and detected by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)in the positive ion electrospray ionization mode.The results showed that the calibration curves for the 16 AGs exhibited good linearity in the concentration range of 25-750 μg/kg with correlation coefficients greater than 0.990.The limits of detection(LOD)were 0.006-0.609 µg/kg and the limits of quantitation(LOQ)were 0.02-1.83 µg/kg.The average recoveries at three spiked levels were in the range of 69.09%-115.50%,with relative standard deviations(RSDs)of 1.04%-16.58%(n=6).The method is sensitive,accurate,and suitable for simultaneous determination of the 16 AGs in beef.关键词
离子对竞争性结合/牛肉/氨基糖苷类兽药/超高效液相色谱-串联质谱Key words
ion pair competitive binding/beef/aminoglycosides/ultra-high performance liquid chromatography-tandem mass spectrometry分类
轻工纺织引用本文复制引用
刘华文,梁飞燕,辛丽娜,李名路..基于离子对竞争性结合作用检测牛肉中16种氨基糖苷类兽药残留[J].肉类研究,2024,38(8):42-47,6.基金项目
广西壮族自治区市场监督管理局科技计划项目(GXSJKJ2021-3 （）
GXSJKJ2024-15) （）
