利用双重实时荧光重组酶辅助扩增技术快速检测肉制品中鼠源性成分OA北大核心CSTPCD
Rapid Detection of Mouse-Derived Components in Meat Products by Duplex Real-Time Recombinase-Aided Amplification
为实现肉制品中大鼠和小鼠源性成分的同步检测,基于实时荧光重组酶辅助扩增(real-time recombinase-aided amplification,Rt-RAA)技术,以大鼠COX3基因和小鼠16S rRNA基因序列保守区域为靶标,分别设计相应的Rt-RAA引物和探针,通过反应参数的优化确定最佳双重Rt-RAA检测反应条件.进一步对建立的双重Rt-RAA检测方法进行特异性、灵敏度及稳定性评估,并应用该方法对模拟市售肉制品进行检测.结果表明…查看全部>>
In this study,real-time recombinase-aided amplification(Rt-RAA)was used for the synchronous detection of rats-and mice-derived components in meat products.Primers and probes targeting the conserved sequences of the COX3 gene in rats and those of the 16S rRNA gene in mice were designed.The reaction parameters were optimized to establish the optimal reaction conditions for duplex Rt-RAA detection.Furthermore,the specificity,sensitivity and stability of the est…查看全部>>
刘明明;周朝旭;颜海燕;田碧英;李根容
重庆市计量质量检测研究院,国家农副加工产品及调味品质量检验检测中心,重庆 400020重庆市计量质量检测研究院,国家农副加工产品及调味品质量检验检测中心,重庆 400020重庆市计量质量检测研究院,国家农副加工产品及调味品质量检验检测中心,重庆 400020重庆市计量质量检测研究院,国家农副加工产品及调味品质量检验检测中心,重庆 400020重庆市计量质量检测研究院,国家农副加工产品及调味品质量检验检测中心,重庆 400020
轻工业
大鼠小鼠肉制品实时荧光重组酶辅助扩增技术肉制品掺假
ratsmicemeat productsreal-time recombinase-aided amplificationmeat product adulteration
《肉类研究》 2024 (9)
15-20,6
重庆市市场监督管理局科研计划项目(CQSJKJDW2023006)
评论