肉类研究2024，Vol.38Issue(9)：15-20,6.DOI:10.7506/rlyj1001-8123-20240522-122
利用双重实时荧光重组酶辅助扩增技术快速检测肉制品中鼠源性成分
Rapid Detection of Mouse-Derived Components in Meat Products by Duplex Real-Time Recombinase-Aided Amplification
摘要
Abstract
In this study,real-time recombinase-aided amplification(Rt-RAA)was used for the synchronous detection of rats-and mice-derived components in meat products.Primers and probes targeting the conserved sequences of the COX3 gene in rats and those of the 16S rRNA gene in mice were designed.The reaction parameters were optimized to establish the optimal reaction conditions for duplex Rt-RAA detection.Furthermore,the specificity,sensitivity and stability of the established duplex Rt-RAA method were evaluated,and it was applied to simulated commercial meat products.The results showed that this method had strong specificity and stability.The detection limits for rats-and mice-derived components were 0.5%and 0.2%,respectively.The detection limits for their synchronous detection were both 0.5%,and the detection limits for synchronous detection of rats-and mice-derived components mixed in simulated commercial meat samples were still as low as 0.5%.Using duplex Rt-RAA and real-time fluorescence quantitative polymerase chain reaction(real-time PCR),highly consistent results were obtained for commercially available samples and simulated samples.Therefore,the duplex Rt-RAA method is suitable for the detection of rats-and mice-derived components in meat products,providing a fast,efficient,and accurate new method for the detection of meat product adulteration.关键词
大鼠/小鼠/肉制品/实时荧光重组酶辅助扩增技术/肉制品掺假Key words
rats/mice/meat products/real-time recombinase-aided amplification/meat product adulteration分类
轻工纺织引用本文复制引用
刘明明,周朝旭,颜海燕,田碧英,李根容..利用双重实时荧光重组酶辅助扩增技术快速检测肉制品中鼠源性成分[J].肉类研究,2024,38(9):15-20,6.基金项目
重庆市市场监督管理局科研计划项目(CQSJKJDW2023006) （CQSJKJDW2023006）
