食品科学2024，Vol.45Issue(19)：26-40,15.DOI:10.7506/spkx1002-6630-20231104-027
不同pH值环境下β-乳球蛋白与咖啡中3种主要多酚之间的相互作用
Interactions between β-Lactoglobulin and Three Major Polyphenols in Coffee under Different pH Environments
摘要
Abstract
The purpose of this study was to explore the non-covalent interactions between three major polyphenols(chlorogenic acid,caffeic acid and ferulic acid)in coffee and β-lactoglobulin(β-LG)at different pH values and their effects on protein structure by multispectroscopic techniques and molecular docking.The results showed that all three coffee polyphenols statically quenched the fluorescence of β-lactoglobulin under two pH conditions.At pH 7.4 and 298 K,the quenching constants(Ksv of chlorogenic acid,caffeic acid and ferulic acid for β-lactoglobulin were 6.53 × 104,3.16 × 104 and 3.09 × 104 L/mol with 1.20,1.02 and 1.14 binding sites and energy transfer efficiency of 34.55％,24.56％and 21.35％,respectively.At pH 3.0 and 298 K,the Ksv were 7.18 × 104,5.24 × 104 and 7.12 × 104 L/mol with 1.28,1.18 and 1.25 binding sites and energy transfer efficiency of 34.70％,30.42％and 29.65％,respectively.The results of circular dichroism showed that the α-helix content of β-LG decreased and the β-sheet content increased at pH 7.4,and the β-sheet content increased and the random coil content decreased at pH 3.0.The results of molecular docking showed that the three polyphenols could bind to the hydrophobic pocket of β-lactoglobulin mainly through hydrogen bonds,van der Waals force and hydrophobic interaction.This study preliminarily revealed the interaction mechanism between β-lactoglobulin and chlorogenic acid,caffeic acid and ferulic acid in coffee milk beverage under different pH conditions.关键词
多酚/β-乳球蛋白/非共价相互作用/多光谱技术/分子对接Key words
polyphenol/β-lactoglobulin/non-covalent interaction/multispectroscopic techniques/molecular docking分类
轻工业引用本文复制引用
翟晓宇,董文江,于鑫欣,胡荣锁..不同pH值环境下β-乳球蛋白与咖啡中3种主要多酚之间的相互作用[J].食品科学,2024,45(19):26-40,15.基金项目
云南省重大科技专项计划项目(202302AE090004) （202302AE090004）
中央级公益性科研院所基本科研业务费专项(1630142022010) （1630142022010）
中国热带农业科学院国家热带农业科学中心创新研究团队项目(CATASCXTD202404) （CATASCXTD202404）
