食品科学2024，Vol.45Issue(19)：87-93,7.DOI:10.7506/spkx1002-6630-20240201-009
海湾扇贝抗氧化肽对酒精性肝损伤的保护作用
Protective Effect of Antioxidant Peptides from Argopecten irradians against Alcoholic Liver Injury
摘要
Abstract
Objective:To investigate the antioxidant effect of peptides from Argopecten irradians and its protective effect and mechanism against ethanol-induced alcoholic liver injury in mice.Methods:A.irradians peptides were prepared by two-step enzymatic hydrolysis followed by membrane separation.The in vitro antioxidant activity of A.irradians peptides,the activating effects on superoxide dismutase(SOD)and alcohol dehydrogenase(ADH)and the potential to promote alcohol metabolism were evaluated.The protective effect and mechanism against alcoholic liver injury in mice were investigated by measuring the activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in the serum,and the activities of ADH,acetaldehyde dehydrogenase(ALDH),glutathione peroxidase(GSH-Px),catalase(CAT)and SOD and malondialdehyde(MDA)content in the liver of a mouse model of acute alcoholism.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)was used to analyze the peptide sequences.PeptideRanker and AnOxPePred 1.0 tools were used to predict the potential biological activity of peptides and conduct molecular docking analysis of the peptide with the strongest antioxidant activity to ALDH.Results:Peptides with molecular mass between 200 and 1 000 Da constituted approximately 70.38％of the total peptides.Antioxidant amino acids accounted for 68.75％of the total amino acids.In vitro experiments showed that the half maximal inhibitory concentration(IC50)values of A.irradians peptides were 19.43,4.53 and 2.19 mg/mL in 1,1-diphenyl-2-picrylhydrazyl(DPPH)and hydroxyl radical scavenging and Fe2+chelating assays,respectively.At 5 mg/mL,the hydrolysate of A.irradians activated SOD and ADH by 19.83％and 18.18％,respectively,demonstrating good antioxidant activity and anti-alcoholic potential.In vivo experimental results showed that A.irradians peptides significantly reduced the levels of ALT and AST in mouse serum,enhanced the activity of ADH,ALDH,GSH-Px,SOD and CAT,and reduced the content of MDA.Using PeptideRanker and AnOxPePred 1.0 tools,44 identified peptides were screened,and 6 of them met the criteria for molecular docking screening.Molecular docking revealed that they had strong binding capacity to ALDH.Among them,DQPHFPF and YSTHPHF formed 7 and 3 hydrogen bonds with ALDH,respectively,which could be activated through molecular interactions with ALDH.Conclusion:This study provides a theoretical reference for the application of peptides products from A.irradians for curing alcohol and protecting liver.关键词
海湾扇贝/活性肽/酒精性肝损伤/氧化应激/分子对接Key words
Argopecten irradians/active peptides/alcoholic liver injury/oxidative stress/molecular docking分类
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曹燕峰,常立炀,张修正,陈娜,蔡芳瑗,张志芹,刘海梅,赵芹..海湾扇贝抗氧化肽对酒精性肝损伤的保护作用[J].食品科学,2024,45(19):87-93,7.基金项目
烟台市科技计划项目(2022XDRH012) （2022XDRH012）
鲁东大学-烟台味禧食品科技有限公司校企合作项目(28000103) （28000103）
烟台市重大科技创新工程项目(2023YT06000829) （2023YT06000829）
