食品科学2024，Vol.45Issue(19)：112-122,11.DOI:10.7506/spkx1002-6630-20240204-029
非靶向代谢组学揭示南昆山毛叶茶绿茶和红茶的独特化学成分组成
Non-targeted Metabolomics Reveals the Unique Chemical Composition of Green Tea and Black Tea from Camellia ptilophylla
摘要
Abstract
To reveal the chemical composition profile of Camellia ptilophylla,a rare natural low-caffeine tea variety,non-targeted metabolomics based on high-resolution mass spectrometry(HR-MS)was employed to identify the metabolite composition of green tea and black tea from C.ptilophylla and C.sinensis var.assamica.Additionally,the metabolomic differences between the two types of tea and between the two tea varieties were analyzed using principal component analysis(PCA),cluster analysis(CA),and orthogonal partial least squares-discriminant analysis(OPLS-DA).Differential metabolites were identified using standards and database.A total of 152 metabolites were identified in the four tea samples,the major ones being flavonoids(42％).These metabolites were categorized into four clusters.The metabolomic difference between C.ptilophylla and C.sinensis var.assamica was more pronounced than that between green and black teas.Notably,24 metabolites including theasinensin B isomers,theobromine and 14 flavonoids such as tetrahydroxyxanthone and epitheaflagallin 3-O-gallate,which were more abundant in C.ptilophylla green tea than in C.sinensis green tea,were considered as the characteristic metabolites of C.ptilophylla green tea.In black tea from C.ptilophylla,40 characteristic metabolites were identified including 25 flavonoids such as gallocatechin-3,5-digallate,tetrahydroxyxanthone and dihydromyricetin;4 tannins such as theaflavin 3-gallate,theasinensin A isomers and theaflavin 3,3'-digallate;and theobromine.This study provides a reference for further exploration of bioactive compounds in C.ptilophylla.关键词
南昆山毛叶茶/绿茶/红茶/非靶向代谢组学/化学成分组成Key words
Camellia ptilophylla/green tea/black tea/non-targeted metabolomics/chemical composition分类
化学化工引用本文复制引用
龚兴鑫,严毅鹏,吕敏,李斌,陈忠正,张媛媛,林晓蓉..非靶向代谢组学揭示南昆山毛叶茶绿茶和红茶的独特化学成分组成[J].食品科学,2024,45(19):112-122,11.基金项目
广东省自然科学基金青年提升项目(2023A1515030190) （2023A1515030190）
广东省自然科学基金面上项目(2022A1515011080) （2022A1515011080）
农业农村部农业科技创新能力条件建设项目(2020-440100-012846) （2020-440100-012846）
