食品科学2024，Vol.45Issue(19)：130-140,11.DOI:10.7506/spkx1002-6630-20240325-182
基于超高效液相色谱-串联质谱技术分析'福红'李冷藏期间初生代谢物动态变化规律
Analysis of Dynamic Changes in Primary Metabolites of'Fuhong'Plum Fruits during Refrigeration by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
To understand the composition and dynamic changes of primary metabolites in'Fuhong'plum fruits during refrigeration,the primary metabolite profile of'Fuhong'plum fruits stored at 4 ℃ for 0,30,and 60 days was analyzed using ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Differential metabolites were selected by orthogonal partial least squares-discriminant analysis(OPLS-DA)and subjected to pathway enrichment analysis.The results showed that a total of 573 metabolites were identified,including sugars,organic acids,amino acids and their derivatives,lipids,and nucleotides.There was a significant difference in primary metabolite profile between the three storage times,and 95 differential metabolites at day 30 vs.0,99 differential metabolites at day 60 vs.30,and 173 differential metabolites at day 60 vs.0 were identified.The pathway enrichment analysis showed that the major metabolic pathways with a significant difference included linoleic acid metabolism,unsaturated fatty acid biosynthesis,propionic acid metabolism,sulfur metabolism,purine metabolism,nucleotide metabolism,cysteine and methionine metabolism.This study provides a theoretical basis for the quality evaluation and postharvest preservation of'Fuhong'plum fruits.关键词
'福红'李/冷藏/超高效液相色谱-串联质谱技术/初生代谢物Key words
'Fuhong'plum/refrigeration/ultra-high performance liquid chromatography-tandem mass spectrometry/primary metabolites分类
轻工业引用本文复制引用
林炎娟,叶新福,方智振,周丹蓉..基于超高效液相色谱-串联质谱技术分析'福红'李冷藏期间初生代谢物动态变化规律[J].食品科学,2024,45(19):130-140,11.基金项目
福建省科技厅对外合作项目(202210032) （202210032）
福建省农业科学院东西部协作项目(DKBF-2023-05) （DKBF-2023-05）
5511协同创新工程项目(KXXYJBG2021006) （KXXYJBG2021006）
