食品科学2024，Vol.45Issue(19)：208-215,8.DOI:10.7506/spkx1002-6630-20240219-091
还原型谷胱甘肽诱导溶菌酶界面组装成膜机制及膜性能分析
Formation Mechanism and Performance of Films through Reduced Glutathione-Induced Lysozyme Interface Assembly
摘要
Abstract
In this study,the mechanism of film formation through glutathione-induced lysozyme interface assembly was verified by spectroscopic methods,and the formed film was characterized by ellipsometry.N-(l-pyrenyl)maleimide(NPM)staining demonstrated that reduced glutathione broken the disulfide bonds of lysozyme leading to release of free sulfhydryl groups.Together,tryptophan fluorescence spectroscopy and lysozyme activity assay verified that the disulfide bond Cys6-Cys127 in lysozyme was broken.In addition,the unfolded lysozyme was found to still have antibacterial activity.The lysozyme film was transparent and colorless.Atom force microscopy(AFM)and transmission electron microscopy(TEM)revealed that it was composed of lysozyme aggregates with a uniform,smooth and dense surface.The results of X-ray photoelectron spectroscopy(XPS)and laser confocal Raman spectroscopy(LCRS)indicated that the lysozyme nanofilm might possess interfacial adhesion.The results of ellipsometry showed that the maximum thickness of the film was approximately 900 nm,and did not increase without limit with the increase in incubation time or the concentration of the reactants.Its stiffness value,elastic modulus and hardness were 29.32-34.21 μN/nm,24.00-27.93 GPa,and 0.44-0.49 GPa,respectively,which were in the strength range of the protein film.The three-phase contact angle of the film was around 70°,indicating good hydrophilicity.Its thermal stability was high,and no degradation occurred at 200 ℃.The preparation of the lysozyme nanofilm provides a good idea and reference for the construction of novel edible coatings.关键词
还原型谷胱甘肽/溶菌酶/解折叠/界面组装/膜Key words
reduced glutathione/lysozyme/unfolding/self-assembly/film分类
轻工纺织引用本文复制引用
周彬,李俊娇,郭大山,武璨,胡煜莹,崔冰..还原型谷胱甘肽诱导溶菌酶界面组装成膜机制及膜性能分析[J].食品科学,2024,45(19):208-215,8.基金项目
国家自然科学基金面上项目(32172354) （32172354）
湖北工业大学绿色工业科技引领计划-自主探索计划项目(XJ2021004401) （XJ2021004401）
