食品科学2024，Vol.45Issue(19)：227-234,8.DOI:10.7506/spkx1002-6630-20240226-136
碳化钒免疫层析法检测玉米中T-2毒素
Development of Vanadium Carbide-Based Immunochromatographic Assay for T-2 Toxin Detection in Maize Samples
摘要
Abstract
The purpose of this study was to construct a vanadium carbide-based immunochromatographic assay for the sensitive detection of T-2 toxin in maize.Vanadium carbide nanosheets(VCNSs)were synthesized by ultrasonic-assisted liquid phase exfoliation,and conjugated with T-2 monoclonal antibody(T-2-mAb)as a signal probe.Based on the competition principle,the T-2 toxin in samples competed with the T-2 antigen on the test(T)line for binding to the black VCNSs-mAb probe.As a result,the T-line value was inversely proportional to T-2 toxin concentration.The best experimental conditions and the standard curve were established based on color development in the T-line of the blank test strip(signal value＞800,detected using an MD-600 reader)and the inhibition rate of 0.5 and 1.5 ng/mL T-2 toxin.The results showed that the linear range of the proposed method was between 0.14 and 0.78 ng/mL,the visual detection limit was 0.25 ng/mL,the cut-off value was 1.5 ng/mL,and the half maximal inhibitory concentration(IC50)was 0.344 ng/mL.The sensitivity was 6.6 times higher than that of the traditional colloidal gold test strip.In addition,the assay did not cross-react with five other common mycotoxins.Moreover,the recoveries for real maize samples ranged from 92.8％to 117.2％with coefficients of variation(CV)of less than 9.13％,and the detection limit of 5.06 μg/kg.In summary,this method is characterized by simple operation,rapid sensitivity and low cost,which enables on-site rapid detection of T-2 toxin in cereals.关键词
T-2毒素/玉米/碳化钒/免疫层析试纸条Key words
T-2 toxin/maize/vanadium carbide/immunochromatographic test strip分类
轻工纺织引用本文复制引用
罗昌伟,陈晓阳,黄现青,宋莲军,张西亚,补彤..碳化钒免疫层析法检测玉米中T-2毒素[J].食品科学,2024,45(19):227-234,8.基金项目
国家自然科学基金青年科学基金项目(32302208) （32302208）
中国博士后科学基金资助项目(2023M741056) （2023M741056）
2022年河南省研究生联合培养基地项目(YJS2022JD16) （YJS2022JD16）
河南省高校科技创新团队支持计划资助项目(23IRTSTHN023) （23IRTSTHN023）
