食品科学2024，Vol.45Issue(19)：242-250,9.DOI:10.7506/spkx1002-6630-20240121-184
基于还原氧化石墨烯-聚苯胺/纳米金复合纳米材料的阻抗型电化学适配体传感器检测啶虫脒
An Impedimetric Aptamer Sensor Based on Reduced Graphene Oxide-Polyaniline/Gold Nanoparticle Nanocomposite for the Detection of Acetamiprid
摘要
Abstract
In this study,a highly sensitive and selective electrochemical aptamer sensor(6-mercapto-l-hexanol(MCH)/Apt/AuNPs/rGO-PANi)for acetamiprid(ACE)was prepared based on Au nanocomposites(AuNPs)/reduced graphene oxide(rGO)-polyaniline(PANi).The morphology and structure of the synthesized nanomaterial and the interface of different electrodes were characterized by transmission electron microscopy(TEM),X-ray diffraction(XRD)and scanning electron microscopy(SEM)/energy dispersive X-ray spectrometry(EDX).The electrochemical properties of different electrodes were studied by cyclic voltammetry and electrochemical impedance spectroscopy,and it was found that the AuNPs/rGO-PANi modified electrode showed a large electrochemical active area and excellent electrochemical properties,which could provide an ideal platform for aptamer fixation and electrochemical sensing of ACE.Under optimal conditions,the linear range of MCH/Apt/AuNPs/rGO-PANi for ACE detection was 1.0-100 000.0 ng/L,and the detection limit was 0.15 ng/L at a signal-to-noise(RSN)of 3.A 100-fold excess of common co-existing pesticides did not significantly interfere with the detection of ACE.The prepared aptamer sensor was applied to the detection of cucumbers and sweet potato leaves with recoveries ranging from 94.5％to 98.5％.The accuracy of the developed method was verified by liquid chromatography-mass spectrometry(LC-MS).关键词
适配体传感器/啶虫脒/电化学阻抗法/食品分析Key words
aptamer sensor/acetamiprid/electrochemical impedance method/food analysis分类
轻工纺织引用本文复制引用
罗丽红,周玉玲,牙禹,蒋翠文,劳水兵,覃国新,闫飞燕,梁钟丹,韦宇宁,李慧玲,殷玮琰..基于还原氧化石墨烯-聚苯胺/纳米金复合纳米材料的阻抗型电化学适配体传感器检测啶虫脒[J].食品科学,2024,45(19):242-250,9.基金项目
广西自然科学基金项目(2023GXNSFAA026442) （2023GXNSFAA026442）
广西农业科学院基本科研业务专项(桂农科2024YP113 （）
桂农科2023YM117) （）
广西农业科学院稳定资助科研团队项目(桂农科2021YT138) （桂农科2021YT138）
广西农业科学院优势学科团队项目(桂农科2021YT136) （桂农科2021YT136）
科技先锋队"强农富民""六个一"专项行动(桂农科盟202416) （桂农科盟202416）
国家市场监管重点实验室(热带果蔬质量与安全)基础应用及重点研究课题(KF-2023001) （热带果蔬质量与安全）
