食品科学2024，Vol.45Issue(19)：263-271,9.DOI:10.7506/spkx1002-6630-20240131-281
蛋白质工程提升糖酶热稳定性研究进展
Research Advances in Protein Engineering to Enhance the Thermal Stability of Glycosidase
摘要
Abstract
Glycosidase is an indispensable enzyme in the sugar industry.It is difficult to maintain the stability of natural glycosidase during long-term high-temperature sugar conversion,limiting its application in industrial production.Numerous studies have shown that protein engineering is an important means for enhancing the thermal stability of glycosidase.This article systematically reviews the latest advances in using protein engineering to improve the thermal stability of glycosidase,and dissects the directed evolution,rational design,and semi-rational design strategies used in protein engineering.It also evaluates the effectiveness of these strategies in enhancing the thermal stability of glycosidase and their potential industrial application value,and presents the advantages,limitations and challenges of each strategy.Finally,it suggests that different strategies should be jointly used to improve the thermal stability of glycosidase and gives an outlook on future research directions with a view to promoting the synergistic improvement of the thermal stability and activity of glycosidase.关键词
糖酶/热稳定性/蛋白质工程/理性设计/半理性设计Key words
glycosidases/thermal stability/protein engineering/rational design/semi-rational design分类
生物科学引用本文复制引用
吴晓怡,吴昊,杨灿,文诗雨,段艳婷,谭文芝,谢雨菲,程云辉,文李..蛋白质工程提升糖酶热稳定性研究进展[J].食品科学,2024,45(19):263-271,9.基金项目
国家自然科学基金青年科学基金项目(32201963) （32201963）
湖南省自然科学基金青年项目(2023JJ40018) （2023JJ40018）
湖南省科技创新计划项目(2023RC3137) （2023RC3137）
湖南省教育厅资助科研项目(22C0137) （22C0137）
