生物技术通报2024，Vol.40Issue(9)：113-122,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0514
马铃薯块茎变绿中StHY5对龙葵素合成的促进作用
Promotion of StHY5 in the Synthesis of SGAs during Tuber Turning-green of Potato
摘要
Abstract
[Objective]Post-harvest storage,transportation,shelving,and other factors can lead to the"turning green"of potato,resulting in a significant accumulation of SGAs in tuber.HY5 plays a crucial role as a key mediator of light signals in plants.Investigating its involvement in the turning-green of tuber can establish a molecular foundation for understanding SGAs accumulation induced by light in post-harvest potato.[Method]Through comprehensive gene expression analysis,transcriptome profiling,targeted metabolite analysis,subcellular localization studies,yeast single hybridization and double luciferase experiments,the involvement of StHY5 in tuber turning green following light exposure was preliminarily elucidated.[Result]The StHY5 gene was expressed in both the peel and flesh of potato,but with different expression patterns.During the treatment of potato greening,the expression of the StHY5 gene in potato meat correlated with changes in SGAs content,while the expression of SGAs synthesizing genes StSGT1/GAME1 and StGAME4 was significantly up-regulated.Yeast single hybridization and double luciferase experiments demonstrated that StHY5 directly up-regulated the expression of StSGT1/GAME1 and StGAME4.[Conclusion]During the process of potato greening,the StHY5 plays a role in promoting the accumulation of SGAs by directly up-regulating the expression of StSGT1/GAME1 and StGAME4.This finding provides a solid foundation for further investigation into the function of StHY5 in potato greening.关键词
马铃薯/StHY5/光照诱导薯块绿变/龙葵素积累Key words
potato/StHY5 gene/tuber turning-green induced by light/SGAs accumulation引用本文复制引用
王超,白如仟,管俊梅,罗稷林,何雪姣,迟绍轶,马玲..马铃薯块茎变绿中StHY5对龙葵素合成的促进作用[J].生物技术通报,2024,40(9):113-122,10.基金项目
国家自然科学基金项目(32360757),云南省自然科学基金项目(202201AT070037),云南省院士工作站项目(202105AF150028),云南省教育厅科研基金研究生项目(22Y184) （32360757）
