生物技术通报2024，Vol.40Issue(9)：225-237,13.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0313
铅污染土壤中解磷菌对玉米根际土壤性质和微生物群落结构的影响
Effects of Phosphate-solubilizing Bacteria on the Rhizosphere Soil Properties and Microbial Community Structure of Maize in Lead-contaminated Soil
摘要
Abstract
[Objective]To explore the effects of phosphate-solubilizing bacteria(PSB)and their fermentation products on the properties of maize(Zea mays L.)rhizosphere soil and the composition and diversity of microbial community in lead-contaminated soil.[Method]Based on the screening of Klebsiella pasteurii with lead-resistant and phosphate-solubilizing function,corn was cultivated in lead-contaminated soil by pot experiment.LB medium,supernatant(bacterial secretion),bacterial solution(only bacterial cells)and fermentation broth(supernatant+bacterial cells)were applied to the rhizosphere,and sterile water control was set to explore the effects of phosphate-solubilizing bacteria on the physical and chemical properties of rhizosphere soil and microbial community structure.[Result]The supernatant,bacterial liquid and fermentation liquid of K.baumannii had no significant effect on the diversity of bacterial community in maize rhizosphere,while the bacterial liquid significantly increased the Shannon index and Chao index of soil fungal community.The supernatant,bacterial liquid and fermentation liquid of the strain increased the relative abundances of heavy metal-resistant microbial groups such as Bacteroidetes and Actinobacteria,while the supernatant and fermentation liquid increased the abundances of Proteobacteria and Mortierellomycota.The supernatant increased the relative abundances of Sphingomonas,Blastococcus,Bradyrhizobium and Archaeorhizomyces.In addition,Pearson correlation analysis of differential genera in the maize rhizosphere soil showed that there were positive correlations among 7 groups of differential genera,which revealed that different microbial genera tended to form mutually beneficial symbiotic relationships.The supernatant,bacterial liquid and fermentation liquid of the strain significantly increased the activities of soil acid phosphatase(Acp).Among them,the Acp activity of maize rhizosphere soil in the fermentation liquid treatment group was the highest(574.44 mg/g,24-1).The application of supernatant and bacterial liquid significantly increased the content of alkali-hydrolyzed nitrogen(AN)in the rhizosphere soil,which was 47.4%and 39.5%higher than that of the control,respectively.The three treatment groups significantly reduced the soil pH value.Through redundancy analysis(RDA),it was found that soil AN,Acp,pH value and available phosphorus(AP)were the main factors affecting the microbial community structure.[Conclusion]This study reveales that exogenous application of PSB and their fermentation products was beneficial to improving the fertility of lead-contaminated soil,and affectes the composition and structure of soil microbial community,which provides a theoretical basis for inoculating PSB and improving soil nutrients and soil microbial community structure in lead-contaminated farmland.关键词
重金属/污染土壤/解磷菌/微生物群落结构/土壤性质Key words
heavy metals/contaminated soil/phosphate-solubilizing bacteria/microbial community structure/soil properties引用本文复制引用
温绍福,江润海,朱城强,张梅,余小琴,杨杰惠,杨小容,侯秀丽..铅污染土壤中解磷菌对玉米根际土壤性质和微生物群落结构的影响[J].生物技术通报,2024,40(9):225-237,13.基金项目
国家自然科学基金项目(42167009),云南省教育厅科学研究基金项目(2022Y712,2023Y0874),云南省高校联合基金项目(2018FH001-004),云南省教育厅云南高原湖泊-北美五大湖国际联合科技创新团队 （42167009）
