基于WGCNA探讨葛根素对呕吐毒素诱导C6细胞损伤的保护作用OA北大核心CSTPCD

[Objective]The experiment used deoxynivalenol(DON)to construct a C6 cell injury model and analyzed the protective mechanism of puerarin(PUE)in alleviating DON-induced C6 cell injury.[Method]By using RNA sequencing(RNA-seq)and weighted gene co expression network analysis(WGCNA),we aimed to identify the key modules and core target genes,which was most closely related to the alleviation of PUE on DON-induced C6 cell injury.Furthermore,we explored the molecular mechanism of PUE in alleviating DON-induced neurotoxicity.[Result]The PUE significantly inhibited the decrease in the cell viability induced by DON,restored the morphology of damaged C6 cells,and effectively alleviated DON-induced cytotoxicity.The RNA seq and WGCNA results indicated that the Blue module was the most relevant key module to the alleviation of PUE on DON-induced C6 cell injury,and S100a11,Pdia3 and Hsp90b1 genes were selected as the key core genes.[Conclusion]These results suggest that PUE may alleviate DON-induced C6 cell injury by targeting key core genes and regulating in endoplasmic reticulum signaling pathway.