生物技术通报2024，Vol.40Issue(9)：301-310,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0130
基于WGCNA探讨葛根素对呕吐毒素诱导C6细胞损伤的保护作用
Exploring the Protective Effect of Puerarin on Deoxynivalenol-induced C6 Cell Injury Based on WGCNA
摘要
Abstract
[Objective]The experiment used deoxynivalenol(DON)to construct a C6 cell injury model and analyzed the protective mechanism of puerarin(PUE)in alleviating DON-induced C6 cell injury.[Method]By using RNA sequencing(RNA-seq)and weighted gene co expression network analysis(WGCNA),we aimed to identify the key modules and core target genes,which was most closely related to the alleviation of PUE on DON-induced C6 cell injury.Furthermore,we explored the molecular mechanism of PUE in alleviating DON-induced neurotoxicity.[Result]The PUE significantly inhibited the decrease in the cell viability induced by DON,restored the morphology of damaged C6 cells,and effectively alleviated DON-induced cytotoxicity.The RNA seq and WGCNA results indicated that the Blue module was the most relevant key module to the alleviation of PUE on DON-induced C6 cell injury,and S100a11,Pdia3 and Hsp90b1 genes were selected as the key core genes.[Conclusion]These results suggest that PUE may alleviate DON-induced C6 cell injury by targeting key core genes and regulating in endoplasmic reticulum signaling pathway.关键词
呕吐毒素/葛根素/加权基因共表达网络分析/C6细胞Key words
deoxynivalenol/puerarin/weighted gene co-expression network analysis(WGCNA)/C6 cell引用本文复制引用
赵海平,刘林,王昕璐,岳鹏飞,孔维军,王蒙..基于WGCNA探讨葛根素对呕吐毒素诱导C6细胞损伤的保护作用[J].生物技术通报,2024,40(9):301-310,10.基金项目
国家自然科学基金项目(32372453),北京市农林科学院杰出科学家培育专项项目(JKZX202403),江西中医药大学现代中药制剂教育部重点实验室开放基金资助项目(zdsys-202109) （32372453）
