中国食品添加剂2024，Vol.35Issue(10)：172-178,7.DOI:10.19804/j.issn1006-2513.2024.10.021
玉米多糖对阿霉素诱导心肌炎症损伤的改善作用及机制研究
Improvement effect and mechanism study of corn polysaccharide on myocardium inflammation induced by doxorubicin
摘要
Abstract
In order to investigate the effects and mechanisms of corn polysaccharide(CPA)on doxorubicin(DOX)-induced myocardial inflammatory damage,acute myocardial injury models in C57 mice were prepared through intraperitoneal injection of DOX.Mice cardiac function was detected by echocardiography,and the ratio of heart weight to body weight was recorded.Serum levels of cardiac enzymes and antioxidant enzyme activities were measured,and myocardial tissue changes were observed using HE staining.The MTT method was utilized to determine the viability of H9c2 cardiomyocytes treated with CPA.ELISA was used to measure the levels of TNF-α,IL-6,and IL-10 in myocardial tissue and cells.Western blot was used to detect the protein levels of NLRP3 and Cleaved caspase-1.The results showed that compared to the normal group,the DOX group exhibited elevated cardiac enzymes and noticeable myocardial tissue damage.The CPA+DOX group showed lower levels of myocardial tissue damage compared to the DOX group.The DOX group displayed a reduced survival rate of H9c2 myocardial cells,while the CPA+DOX group showed significantly elevated levels of anti-inflammatory cytokines compared to the DOX group,and the levels of NLRP3,caspase-1,and IL-1β in cells and tissues were reduced by the cleavage of translated proteins.It was concluded that CPA demonstrated an ability to mitigate the impact of DOX-induced cardiac toxicity in both in vivo and in vitro experiments.关键词
玉米多糖/阿霉素/NLRP3炎症小体/心肌损伤Key words
corn polysaccharide/doxorubicin/NLRP3 inflammation/myocardial injury分类
轻工纺织引用本文复制引用
夏晓培,马广礼,赵静,邓明珠,刘翠翠..玉米多糖对阿霉素诱导心肌炎症损伤的改善作用及机制研究[J].中国食品添加剂,2024,35(10):172-178,7.基金项目
许昌市哲学社会科学规划课题(xkt202204340) （xkt202204340）
